{"doi":"10.17504/protocols.io.4pfgvjn","title":"HTAPP_NST- Nuclei isolation from frozen tissue v1","abstract":"<p>This protocol describes a nuclei isolation method for frozen tissues based on the previously published protocol by Gao et al. and modified Drokhlyansky et al (unpublished). This protocol is compatible with 10X droplet-based single-cell technology.   This method is part of a toolbox for processing frozen tissue samples, including the NST (this protocol), CST and TST protocols (all available in protocols.io). We recommend users to test all three protocols on their tissue type of interest, and perform side-by-side comparison of the data generated.  For the HTAPP project, this protocol was tested on metastatic breast cancer, ovarian cancer and pediatric neuroblastoma frozen samples. For these cancer types, however,  TST was the protocol of choice. When tested on HTAPP samples, NST, in general, underperformed compared to TST and CST,  based on several QC parameters.</p>","journal":null,"year":2019,"id":5480,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.0421,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2019-06-24","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":16531,"name":"Nicholas Van Wittenberghe","orcid":"0000-0002-7653-9729","position":1,"is_corresponding":false},{"id":2274,"name":"Michal Slyper","orcid":"0000-0002-3002-0366","position":2,"is_corresponding":false},{"id":2275,"name":"Julia Waldman","orcid":"0000-0002-0584-5833","position":3,"is_corresponding":false},{"id":2697,"name":"Orit Rozenblatt–Rosen","orcid":"0000-0001-6313-3570","position":4,"is_corresponding":false},{"id":29633,"name":"Prisca Liberali","orcid":"0000-0003-0695-6081","position":5,"is_corresponding":false},{"id":3735,"name":"Orit Rozenblatt‐Rosen","orcid":null,"position":6,"is_corresponding":false},{"id":16530,"name":"Eugene Drokhlyansky","orcid":"0000-0002-1939-3559","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}