{"doi":"10.17504/protocols.io.4j9gur6","title":"HTAPP_EZ Nuclei Isolation from frozen tissue v1","abstract":"<p>This protocol describes a nuclei isolation method based on the previously publishedDroNc-seq step-by-step protocol (linkbelow) with some modifications and instructions for loading the nuclei on 10X genomics.  For the HTAPP project, this protocol was tested on pediatric neuroblastoma samples. For this cancer type, however, the TST nuclei isolation method was the protocol of choice. The TST method is part of a toolbox for processing frozen tissue samples, including the TST, CST and TST protocols (all available in protocols.io). The EZ method was developed for brain tissue (link), and when tested on HTAPP samples, it underperformed compared to TST, CST and NST,  based on several QC parameters. We recommend users to test all protocols on their tissue type of interest, and perform side-by-side comparison of the data generated.   </p>","journal":null,"year":2019,"id":11321,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.045,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2019-06-21","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":15891,"name":"Cristin McCabe","orcid":"0000-0002-7209-7847","position":1,"is_corresponding":false},{"id":16530,"name":"Eugene Drokhlyansky","orcid":"0000-0002-1939-3559","position":2,"is_corresponding":false},{"id":2697,"name":"Orit Rozenblatt–Rosen","orcid":"0000-0001-6313-3570","position":3,"is_corresponding":false},{"id":29633,"name":"Prisca Liberali","orcid":"0000-0003-0695-6081","position":4,"is_corresponding":false},{"id":3735,"name":"Orit Rozenblatt‐Rosen","orcid":null,"position":5,"is_corresponding":false},{"id":7418,"name":"Naomi Habib","orcid":"0000-0002-6049-2487","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}