{"doi":"10.17504/protocols.io.261gerpjol47/v1","title":"Manual Uterine Tissue Dissociation for Multiome Analysis v1","abstract":"This protocol was developed in accordance with the mission statement of the Human BioMolecular Atlas Program (HuBMAP) of the NIH, to create an open and global platform to map healthy cells in the human body. Protocols developed through HuBMAP can be used by researchers across the world to create Standard Operating Protocols (SOPs) for sample collection and processing. This protocol describes dissociation of snap-frozen uterine tissues in order to isolate nuclei that can be used for downstream molecular analysis. This protocol can also be used on cell suspensions made from fresh tissue; freezing tissues leads to a slight decrease in RNA quality after tissue thawing and dissociation. If using fresh tissue, we recommend optimizing the lysis time. We use this protocol to generate high quality 10X Multiome libraries from snap-frozen uterine and cervix tissue biopsies.","journal":null,"year":2024,"id":508383,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.7278,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":894330,"name":"Kathleen O’Neill","orcid":"0000-0003-1980-6840","position":1,"is_corresponding":false},{"id":1360346,"name":"Stephen D. Fisher","orcid":"0000-0002-1006-1638","position":2,"is_corresponding":false},{"id":251905,"name":"Junhyong Kim","orcid":"0000-0002-7726-8246","position":3,"is_corresponding":false},{"id":1360495,"name":"Sarah A Johnston","orcid":null,"position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:11:10.049781Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}