{"doi":"10.1681/asn.2004050387","title":"Heparan Sulfate of Perlecan Is Involved in Glomerular Filtration","abstract":"\n \n \n Perlecan is a heparan sulfate proteoglycan and a major component of the glomerular basement membrane. To understand the role of heparan sulfate chains of perlecan in glomerular filtration, detailed analyses were performed of the kidneys of\n \n Hspg2\n \n \n Δ3/Δ3 mice, whose perlecan lacks heparan sulfate attachment sites in N-terminal domain I. Macroscopic, histologic, and electron microscopic observations, as well as immunohistochemical and immunoelectron microscopic analyses using specific antibodies against perlecan and agrin core proteins, revealed no significant abnormalities in these mice under physiologic conditions. Polyethyleneimine staining demonstrated no significant changes in charge density in the glomerular basement membrane. Transcripts of other heparan sulfate proteoglycans, agrin, and collagen type XVIII, as well as perlecan, were expressed at similar levels to those in the wild-type littermates. Approximately 40% of the perlecan synthesized by\n \n Hspg2\n \n \n Δ3/Δ3 fibroblasts was substituted with heparin sulfate and 60% was substituted with chondroitin sulfate. All of the perlecan synthesized by wild-type fibroblasts contained heparin sulfate, indicating an altered substitution of glycosaminoglycans on\n \n Hspg2\n \n \n Δ3/Δ3 perlecan. Immunostaining indicated that the level of chondroitin sulfate was actually increased in the\n \n Hspg2\n \n \n Δ3/Δ3 glomerular basement membrane. When administered intraperitoneally with BSA,\n \n Hspg2\n \n \n Δ3/Δ3 mice exhibited remarkable proteinuria. These findings suggest that heparan sulfate chains of perlecan play an important role in glomerular filtration, especially of a large amount of protein.\n \n ","journal":"Journal of the American Society of Nephrology","year":2005,"id":36900,"datarank":4.813292008607181,"base_score":4.634728988229636,"endowment":4.634728988229636,"self_citation_contribution":0.6952093482344455,"citation_network_contribution":4.118082660372736,"self_endowment_contribution":0.6952093482344455,"citer_contribution":4.118082660372736,"corpus_percentile":null,"corpus_rank":null,"citation_count":102,"citer_count":99,"citers_with_citation_signal":86,"citers_with_endowment":86,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":185997,"name":"Ashio Yoshimura","orcid":null,"position":1,"is_corresponding":false},{"id":185998,"name":"Kiyoko Inui","orcid":null,"position":2,"is_corresponding":false},{"id":185999,"name":"Terukuni Ideura","orcid":null,"position":3,"is_corresponding":false},{"id":186000,"name":"Hideto Watanabe","orcid":null,"position":4,"is_corresponding":false},{"id":105552,"name":"Ling Wang","orcid":"0000-0001-7524-2977","position":5,"is_corresponding":false},{"id":186001,"name":"Raija Soininen","orcid":null,"position":6,"is_corresponding":false},{"id":186002,"name":"Karl Tryggvason","orcid":null,"position":7,"is_corresponding":false},{"id":185996,"name":"Hiroyuki Morita","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.634728988229636,"endowment":4.634728988229636,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"15872080","pmcid":null,"openalex_id":"https://openalex.org/W2048927510","authors":[],"funders":[],"total_grants":0,"fwci":3.9049,"citation_percentile":0.93519346,"influential_citations":5,"citation_trend":[{"year":2012,"count":13},{"year":2013,"count":5},{"year":2014,"count":3},{"year":2015,"count":4},{"year":2016,"count":3},{"year":2017,"count":6},{"year":2018,"count":3},{"year":2019,"count":2},{"year":2020,"count":4},{"year":2021,"count":4},{"year":2022,"count":1},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://journals.lww.com/jasn/fulltext/2005/06000/heparan_sulfate_of_perlecan_is_involved_in.23.aspx","host_type":"BRONZE"},{"url":"https://journals.lww.com/00001751-200506000-00023","host_type":"publisher"},{"url":"https://doi.org/10.1681/asn.2004050387","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/15872080","host_type":"repository"}],"fields_of_study":["Proteoglycans and glycosaminoglycans research","Cell Adhesion Molecules Research","Platelet Disorders and Treatments","Medicine","Chemistry","Biology","Animals","Basement Membrane","Glomerular Filtration Rate","Heparan Sulfate Proteoglycans","Kidney Glomerulus","Mice","Models, Animal"],"mesh_terms":["Animals","Basement Membrane","Glomerular Filtration Rate","Kidney Glomerulus","Heparan Sulfate Proteoglycans","Models, Animal","Mice"],"keywords":["Perlecan","Heparan sulfate","Glomerular basement membrane","Agrin","Chondroitin sulfate","Basement membrane","Chemistry","Heparin","Chondroitin sulfate proteoglycan","Proteoglycan","Type IV collagen","Biochemistry","Glycosaminoglycan","Extracellular matrix","Molecular biology","Cell biology","Endocrinology","Kidney","Biology","Glomerulonephritis","Laminin"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-10T17:48:17.150921Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}