{"doi":"10.1677/joe.0.1640187","title":"Stimulation of glucose transport by thyroid hormone in 3T3-L1 adipocytes: increased abundance of GLUT1 and GLUT4 glucose transporter proteins","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>In 3T3-L1 adipocytes we have examined the effect of tri-iodothyronine (T(3)) on glucose transport, total protein content and subcellular distribution of GLUT1 and GLUT4 glucose transporters. Cells incubated in T(3)-depleted serum were used as controls. Cells treated with T(3) (50 nM) for three days had a 3.6-fold increase in glucose uptake (P&amp;lt;0.05), and also presented a higher insulin sensitivity, without changes in insulin binding. The two glucose carriers, GLUT1 and GLUT4, increased by 87% (P&amp;lt;0.05) and 90% (P&amp;lt;0. 05), respectively, in cells treated with T(3). Under non-insulin-stimulated conditions, plasma membrane fractions obtained from cells exposed to T(3) were enriched with both GLUT1 (3. 29+/-0.69 vs 1.20+/-0.29 arbitrary units (A.U.)/5 microg protein, P&amp;lt;0.05) and GLUT4 (3.50+/-1.16 vs 0.82+/-0.28 A.U./5 microg protein, P&amp;lt;0.03). The incubation of cells with insulin produced the translocation of both glucose transporters to plasma membranes, and again cells treated with T(3) presented a higher amount of GLUT1 and GLUT4 in the plasma membrane fractions (P&amp;lt;0.05 and P&amp;lt;0.03 respectively). These data indicate that T(3) has a direct stimulatory effect on glucose transport in 3T3-L1 adipocytes due to an increase in GLUT1 and GLUT4, and by favouring their partitioning to plasma membranes. The effect of T(3) on glucose uptake induced by insulin can also be explained by the high expression of both glucose transporters.</jats:p>","journal":"Journal of Endocrinology","year":2000,"id":636357,"datarank":0.6064576901751826,"base_score":4.04305126783455,"endowment":4.04305126783455,"self_citation_contribution":0.6064576901751826,"citation_network_contribution":0.0,"self_endowment_contribution":0.6064576901751826,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":56,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1651543,"name":"B Casanova","orcid":null,"position":1,"is_corresponding":false},{"id":1651544,"name":"N Pulido","orcid":null,"position":2,"is_corresponding":false},{"id":1651545,"name":"AI Suarez","orcid":null,"position":3,"is_corresponding":false},{"id":1651546,"name":"E Rodriguez","orcid":null,"position":4,"is_corresponding":false},{"id":1651547,"name":"A Rovira","orcid":null,"position":5,"is_corresponding":false},{"id":1587275,"name":"R Romero","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Stimulation of glucose transport by thyroid hormone in 3T3-L1 adipocytes: increased abundance of GLUT1 and GLUT4 glucose transporter proteins","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>In 3T3-L1 adipocytes we have examined the effect of tri-iodothyronine (T(3)) on glucose transport, total protein content and subcellular distribution of GLUT1 and GLUT4 glucose transporters. Cells incubated in T(3)-depleted serum were used as controls. Cells treated with T(3) (50 nM) for three days had a 3.6-fold increase in glucose uptake (P&amp;lt;0.05), and also presented a higher insulin sensitivity, without changes in insulin binding. The two glucose carriers, GLUT1 and GLUT4, increased by 87% (P&amp;lt;0.05) and 90% (P&amp;lt;0. 05), respectively, in cells treated with T(3). Under non-insulin-stimulated conditions, plasma membrane fractions obtained from cells exposed to T(3) were enriched with both GLUT1 (3. 29+/-0.69 vs 1.20+/-0.29 arbitrary units (A.U.)/5 microg protein, P&amp;lt;0.05) and GLUT4 (3.50+/-1.16 vs 0.82+/-0.28 A.U./5 microg protein, P&amp;lt;0.03). The incubation of cells with insulin produced the translocation of both glucose transporters to plasma membranes, and again cells treated with T(3) presented a higher amount of GLUT1 and GLUT4 in the plasma membrane fractions (P&amp;lt;0.05 and P&amp;lt;0.03 respectively). These data indicate that T(3) has a direct stimulatory effect on glucose transport in 3T3-L1 adipocytes due to an increase in GLUT1 and GLUT4, and by favouring their partitioning to plasma membranes. The effect of T(3) on glucose uptake induced by insulin can also be explained by the high expression of both glucose transporters.</jats:p>","is_dataset_classified":null,"base_score":4.04305126783455,"endowment":4.04305126783455,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"10657854","pmcid":null,"openalex_id":"https://openalex.org/W2051963643","authors":[],"funders":[],"total_grants":0,"fwci":0.2444,"citation_percentile":0.54522364,"influential_citations":0,"citation_trend":[{"year":2012,"count":3},{"year":2014,"count":1},{"year":2015,"count":1},{"year":2016,"count":6},{"year":2017,"count":5},{"year":2018,"count":1},{"year":2022,"count":2},{"year":2023,"count":1},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://joe.bioscientifica.com/downloadpdf/journals/joe/164/2/187.pdf","host_type":"journal"},{"url":"https://joe.bioscientifica.com/downloadpdf/journals/joe/164/2/187.pdf","host_type":"publisher"},{"url":"https://joe.bioscientifica.com/view/journals/joe/164/2/187.xml","host_type":"publisher"},{"url":"https://journals.bioscientifica.com/joe/article-pdf/164/2/187/108003/187.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1677/joe.0.1640187","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/10657854","host_type":"repository"}],"fields_of_study":["Metabolism, Diabetes, and Cancer","Protein Kinase Regulation and GTPase Signaling","Thyroid Disorders and Treatments","3T3 Cells","Adipocytes","Animals","Blotting, Western","Cells, Cultured","Deoxyglucose","Fibroblasts","Glucose Transporter Type 1","Glucose Transporter Type 4","Insulin","Mice","Monosaccharide Transport Proteins","Muscle Proteins","Triiodothyronine"],"mesh_terms":["Animals","Cells, Cultured","Deoxyglucose","Fibroblasts","Insulin","Monosaccharide Transport Proteins","Muscle Proteins","Triiodothyronine","Blotting, Western","3T3 Cells","Adipocytes","Glucose Transporter Type 1","Glucose Transporter Type 4","Mice"],"keywords":["GLUT1","GLUT4","Glucose transporter","Internal medicine","Endocrinology","Glucose Transporter Type 1","Glucose uptake","Insulin","Chemistry","Membrane transport","Stimulation","Biology","Biochemistry","Membrane","Medicine"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T16:53:04.205166Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}