{"doi":"10.1677/joe.0.1290011","title":"Desensitization studies using perifused rat pituitary cells show that growth hormone-releasing hormone and His-d-Trp-Ala-Trp-d-Phe-Lys-NH2 stimulate growth hormone release through distinct receptor sites","abstract":"<jats:title>ABSTRACT</jats:title>\n        <jats:p>The hexapeptide His-<jats:sc>d</jats:sc>-Trp-Ala-Trp-<jats:sc>d</jats:sc>-Phe-Lys-NH<jats:sub>2</jats:sub> (GHRP-6) and GH-releasing factor (GHRH) produced a rapid release of GH upon perifusion of dispersed rat pituitary cells. In contrast to the native hormone GHRH, GHRP-6 elicited a response of short duration. When perifusion of each secretagogue was continued until the cells no longer released GH, a challenge by the alternative secretagogue immediately resulted in a secondary release of GH. These results are consistent with each secretagogue causing desensitization of discrete receptor-linked second messenger pathways. Cells which were perifused for 1 min with GHRP-6 required continued perifusion with culture medium alone for 60 min before they completely regained responsiveness to a subsequent challenge with GHRP-6. Somatostatin (SRIF) was able to inhibit the action of either secretagogue completely. However, when both GHRH and GHRP-6 were perifused together, SRIF attenuated but did not block GH secretion. These perifusion data add support to conclusions derived from static cell culture studies, that GHRH and GHRP-6 act through different receptor sites and that through discrete signalling pathways their individual effects on GH release are amplified</jats:p>\n        <jats:p><jats:italic>Journal of Endocrinology</jats:italic> (1991) <jats:bold>129,</jats:bold> 11–19</jats:p>","journal":"Journal of Endocrinology","year":1991,"id":15184,"datarank":10.895823430576094,"base_score":4.990432586778736,"endowment":4.990432586778736,"self_citation_contribution":0.7485648880168105,"citation_network_contribution":10.147258542559284,"self_endowment_contribution":0.7485648880168105,"citer_contribution":10.147258542559284,"corpus_percentile":null,"corpus_rank":null,"citation_count":146,"citer_count":142,"citers_with_citation_signal":131,"citers_with_endowment":131,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":116866,"name":"R. G. Smith","orcid":null,"position":1,"is_corresponding":false},{"id":116865,"name":"A. D. Blake","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.990432586778736,"endowment":4.990432586778736,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"1674284","pmcid":null,"openalex_id":"https://openalex.org/W2045264853","authors":[],"funders":[],"total_grants":0,"fwci":7.508,"citation_percentile":0.97973124,"influential_citations":7,"citation_trend":[{"year":2012,"count":5},{"year":2013,"count":2},{"year":2015,"count":1},{"year":2017,"count":1},{"year":2025,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://joe.bioscientifica.com/view/journals/joe/129/1/joe_129_1_003.xml","host_type":"publisher"},{"url":"https://joe.bioscientifica.com/downloadpdf/journals/joe/129/1/joe_129_1_003.xml","host_type":"publisher"},{"url":"https://doi.org/10.1677/joe.0.1290011","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/1674284","host_type":"repository"}],"fields_of_study":["Growth Hormone and Insulin-like Growth Factors","Adrenal Hormones and Disorders","Pituitary Gland Disorders and Treatments","Chemistry","Medicine","Biology","Animals","Cells, Cultured","Growth Hormone","Growth Hormone-Releasing Hormone","Hormones","Male","Oligopeptides","Perfusion","Pituitary Gland","Rats","Rats, Inbred Strains","Second Messenger Systems","Somatostatin","Stimulation, Chemical"],"mesh_terms":["Animals","Cells, Cultured","Hormones","Male","Oligopeptides","Perfusion","Pituitary Gland","Rats, Inbred Strains","Somatostatin","Growth Hormone","Growth Hormone-Releasing Hormone","Stimulation, Chemical","Second Messenger Systems","Rats"],"keywords":["Internal medicine","Secretagogue","Endocrinology","Somatostatin","Desensitization (medicine)","Receptor","Growth hormone–releasing hormone","Thyrotropin-releasing hormone receptor","Second messenger system","Peptide hormone","Hormone","Chemistry","Biology","Stimulation","Growth hormone","Hormone receptor","Medicine"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-01T16:43:22.297482Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}