{"doi":"10.1677/joe.0.0590637","title":"STEROID METABOLISM\n                    <scp>IN VITRO</scp>\n                    BY AN INTERSTITIAL CELL TUMOUR AND THE ATTACHED PREPUBERTAL TESTIS","abstract":"<jats:title>SUMMARY</jats:title>\n                  <jats:p>A virilizing interstitial cell tumour and the attached testicular tissue from a 4-year-old boy were incubated in vitro with [7α-3H]pregnenolone and [4-14C]progesterone, or [4-14C]androstenedione and [7α-3H]5α-dihydrotestosterone. Ring A saturated steroids were produced from 4-ene precursors by the prepubertal testis, but this tissue was unable to convert pregnenolone or progesterone to 17α-hydroxylated C21 steroids, or to C19 steroids.</jats:p>\n                  <jats:p>The virilizing interstitial cell tumour metabolized pregnenolone and progesterone to 17α-hydroxyprogesterone, androstenedione and testosterone. In addition, dehydroepiandrosterone was detected as a product of pregnenolone. The tumour lacked 4-ene-5α-steroid reductase activity.</jats:p>\n                  <jats:p>5α-Dihydrotestosterone was metabolized to 5α-androstane-3,17-dione, androsterone, isoandrosterone, 5a-androstane-3α,17β-diol and 5α-androstane-3β,17β-diol in both the normal and tumour tissue.</jats:p>\n                  <jats:p>The significance of these metabolic pathways is discussed.</jats:p>","journal":"Journal of Endocrinology","year":1973,"id":595837,"datarank":0.26876392038420827,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.0,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1525922,"name":"A. MUNRO NEVILLE","orcid":null,"position":1,"is_corresponding":false},{"id":1525921,"name":"GWEN RICHARDS","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"STEROID METABOLISM\n                    <scp>IN VITRO</scp>\n                    BY AN INTERSTITIAL CELL TUMOUR AND THE ATTACHED PREPUBERTAL TESTIS","abstract":"<jats:title>SUMMARY</jats:title>\n                  <jats:p>A virilizing interstitial cell tumour and the attached testicular tissue from a 4-year-old boy were incubated in vitro with [7α-3H]pregnenolone and [4-14C]progesterone, or [4-14C]androstenedione and [7α-3H]5α-dihydrotestosterone. Ring A saturated steroids were produced from 4-ene precursors by the prepubertal testis, but this tissue was unable to convert pregnenolone or progesterone to 17α-hydroxylated C21 steroids, or to C19 steroids.</jats:p>\n                  <jats:p>The virilizing interstitial cell tumour metabolized pregnenolone and progesterone to 17α-hydroxyprogesterone, androstenedione and testosterone. In addition, dehydroepiandrosterone was detected as a product of pregnenolone. The tumour lacked 4-ene-5α-steroid reductase activity.</jats:p>\n                  <jats:p>5α-Dihydrotestosterone was metabolized to 5α-androstane-3,17-dione, androsterone, isoandrosterone, 5a-androstane-3α,17β-diol and 5α-androstane-3β,17β-diol in both the normal and tumour tissue.</jats:p>\n                  <jats:p>The significance of these metabolic pathways is discussed.</jats:p>","is_dataset_classified":null,"base_score":1.791759469228055,"endowment":1.791759469228055,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"4271540","pmcid":null,"openalex_id":"https://openalex.org/W2080798079","authors":[],"funders":[],"total_grants":0,"fwci":0.0,"citation_percentile":0.0957121,"influential_citations":0,"citation_trend":[{"year":2021,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://joe.bioscientifica.com/view/journals/joe/59/3/joe_59_3_025.xml","host_type":"publisher"},{"url":"https://journals.bioscientifica.com/joe/article-pdf/59/3/637/59732/joe_59_3_025.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1677/joe.0.0590637","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/4271540","host_type":"repository"}],"fields_of_study":["Sexual Differentiation and Disorders","Testicular diseases and treatments","Hormonal and reproductive studies","Adolescent","Androstenedione","Carbon Radioisotopes","Chromatography, Thin Layer","Dehydroepiandrosterone","Humans","Hydroxyprogesterones","Leydig Cell Tumor","Male","Pregnenolone","Progesterone","Puberty","Steroids","Testicular Neoplasms","Testis","Testosterone","Tritium"],"mesh_terms":["Adolescent","Androstenedione","Carbon Radioisotopes","Chromatography, Thin Layer","Dehydroepiandrosterone","Humans","Hydroxyprogesterones","Leydig Cell Tumor","Male","Pregnenolone","Progesterone","Puberty","Steroids","Testicular Neoplasms","Testis","Testosterone","Tritium"],"keywords":["Pregnenolone","Androsterone","Androstenedione","Endocrinology","Internal medicine","Dihydrotestosterone","Dehydroepiandrosterone","Testosterone (patch)","Steroid","Chemistry","Androstane","Metabolism","Leydig cell","In vitro","Androstenediol","Androgen","Biology","Medicine","Biochemistry","Hormone","Luteinizing hormone"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-27T17:57:03.529001Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}