{"doi":"10.1677/jme-08-0167","title":"Short term regulation of aldosterone secretion after stimulation and suppression experiments in mice","abstract":"<jats:p>Aldosterone is synthesized acutely from the zona glomerulosa cells upon stimulation by the renin–angiotensin–aldosterone system. Several enzymes are involved in this steroidogenic process including the steroidogenic acute regulatory protein (<jats:italic>StAR</jats:italic>), P450 side chain cleavage enzyme (<jats:italic>Cyp11a1</jats:italic>), and aldosterone synthase (<jats:italic>Cyp11b2</jats:italic>) which has been demonstrated to be transcriptionally regulated by the nuclear transcription factors <jats:italic>NGF1-B</jats:italic> and <jats:italic>Nurr1</jats:italic>. We investigated the short time transcriptional regulation of these genes in wild-type mice at 10 min intervals for 1 h following application of 0.2 nmol angiotensin II (ANGII) or sodium chloride in comparison sham injections. Using real-time PCR a fast upregulation of adrenal <jats:italic>Cyp11b2</jats:italic> expression (53±5% increase over baseline) could be observed 10 min after sham injection which was accompanied by a transient increase in aldosterone secretion while <jats:italic>StAR</jats:italic> and <jats:italic>Cyp11a1</jats:italic> upregulation was delayed and more sustained. ANGII caused an increase of <jats:italic>StAR</jats:italic> and <jats:italic>Cyp11a1</jats:italic> expression similar to that observed after sham injection while <jats:italic>Cyp11b2</jats:italic> upregulation was more pronounced (10 min, 236±39%) and reflected ANGII induced stimulation of aldosterone output. Sodium challenge was followed by a sustained reduction of all three genes examined (<jats:italic>Cyp11b2</jats:italic>, 20 min, −63±6%) which was accompanied by a significant suppression of aldosterone secretion detectable after 60 min. While increases in <jats:italic>NGF1-B</jats:italic> mRNA levels were similar between the treatment groups, <jats:italic>Nurr1</jats:italic> expression levels were induced only upon ANGII administration. These data suggest that acute regulation of aldosterone synthesis is accompanied by fast transcriptional modulation of steroidogenic enzymes and transcription factors that are likely to be involved in aldosterone secretion.</jats:p>","journal":"Journal of Molecular Endocrinology","year":2009,"id":588181,"datarank":1.0175854010262966,"base_score":3.044522437723423,"endowment":3.044522437723423,"self_citation_contribution":0.4566783656585135,"citation_network_contribution":0.5609070353677832,"self_endowment_contribution":0.4566783656585135,"citer_contribution":0.5609070353677832,"corpus_percentile":null,"corpus_rank":null,"citation_count":20,"citer_count":14,"citers_with_citation_signal":11,"citers_with_endowment":11,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1504648,"name":"Jenny Manolopoulou","orcid":null,"position":1,"is_corresponding":false},{"id":1504649,"name":"Sibylle Wagner","orcid":null,"position":2,"is_corresponding":false},{"id":1504650,"name":"Martin Bidlingmaier","orcid":null,"position":3,"is_corresponding":false},{"id":16822,"name":"Martin Reincke","orcid":null,"position":4,"is_corresponding":false},{"id":58340,"name":"Felix Beuschlein","orcid":"0000-0001-7826-3984","position":5,"is_corresponding":false},{"id":239604,"name":"Ariadni Spyroglou","orcid":"0000-0001-9525-2275","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Short term regulation of aldosterone secretion after stimulation and suppression experiments in mice","abstract":"<jats:p>Aldosterone is synthesized acutely from the zona glomerulosa cells upon stimulation by the renin–angiotensin–aldosterone system. Several enzymes are involved in this steroidogenic process including the steroidogenic acute regulatory protein (<jats:italic>StAR</jats:italic>), P450 side chain cleavage enzyme (<jats:italic>Cyp11a1</jats:italic>), and aldosterone synthase (<jats:italic>Cyp11b2</jats:italic>) which has been demonstrated to be transcriptionally regulated by the nuclear transcription factors <jats:italic>NGF1-B</jats:italic> and <jats:italic>Nurr1</jats:italic>. We investigated the short time transcriptional regulation of these genes in wild-type mice at 10 min intervals for 1 h following application of 0.2 nmol angiotensin II (ANGII) or sodium chloride in comparison sham injections. Using real-time PCR a fast upregulation of adrenal <jats:italic>Cyp11b2</jats:italic> expression (53±5% increase over baseline) could be observed 10 min after sham injection which was accompanied by a transient increase in aldosterone secretion while <jats:italic>StAR</jats:italic> and <jats:italic>Cyp11a1</jats:italic> upregulation was delayed and more sustained. ANGII caused an increase of <jats:italic>StAR</jats:italic> and <jats:italic>Cyp11a1</jats:italic> expression similar to that observed after sham injection while <jats:italic>Cyp11b2</jats:italic> upregulation was more pronounced (10 min, 236±39%) and reflected ANGII induced stimulation of aldosterone output. Sodium challenge was followed by a sustained reduction of all three genes examined (<jats:italic>Cyp11b2</jats:italic>, 20 min, −63±6%) which was accompanied by a significant suppression of aldosterone secretion detectable after 60 min. While increases in <jats:italic>NGF1-B</jats:italic> mRNA levels were similar between the treatment groups, <jats:italic>Nurr1</jats:italic> expression levels were induced only upon ANGII administration. These data suggest that acute regulation of aldosterone synthesis is accompanied by fast transcriptional modulation of steroidogenic enzymes and transcription factors that are likely to be involved in aldosterone secretion.</jats:p>","is_dataset_classified":null,"base_score":3.044522437723423,"endowment":3.044522437723423,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19221003","pmcid":null,"openalex_id":"https://openalex.org/W2157914915","authors":[],"funders":[],"total_grants":0,"fwci":1.26,"citation_percentile":0.81230376,"influential_citations":1,"citation_trend":[{"year":2012,"count":2},{"year":2013,"count":2},{"year":2014,"count":2},{"year":2015,"count":2},{"year":2016,"count":2},{"year":2017,"count":2},{"year":2021,"count":1},{"year":2024,"count":1},{"year":2026,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://jme.bioscientifica.com/downloadpdf/journals/jme/42/5/407.pdf","host_type":"journal"},{"url":"https://jme.bioscientifica.com/downloadpdf/journals/jme/42/5/407.pdf","host_type":"BRONZE"},{"url":"https://jme.bioscientifica.com/downloadpdf/journals/jme/42/5/407.pdf","host_type":"publisher"},{"url":"https://jme.bioscientifica.com/view/journals/jme/42/5/407.xml","host_type":"publisher"},{"url":"https://jme.bioscientifica.com/downloadpdf/journals/jme/42/5/407.xml","host_type":"publisher"},{"url":"https://doi.org/10.1677/jme-08-0167","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/19221003","host_type":"repository"},{"url":"https://push-zb.helmholtz-muenchen.de/frontdoor.php?source_opus=189","host_type":"repository"}],"fields_of_study":["Hormonal Regulation and Hypertension","Nuclear Receptors and Signaling","Estrogen and related hormone effects","Chemistry","Biology","Medicine","Aldosterone","Angiotensin II","Animals","Cholesterol Side-Chain Cleavage Enzyme","Cytochrome P-450 CYP11B2","DNA-Binding Proteins","Gene Expression Regulation","Mice","Nuclear Receptor Subfamily 4, Group A, Member 2","Phosphoproteins","RNA, Messenger","Sodium Chloride","Time Factors","Transcription Factors","Transcription, Genetic","Steroidogenic Acute Regulatory Protein"],"mesh_terms":["Steroidogenic Acute Regulatory Protein","Aldosterone","Angiotensin II","Animals","Cholesterol Side-Chain Cleavage Enzyme","DNA-Binding Proteins","Gene Expression Regulation","Phosphoproteins","RNA, Messenger","Sodium Chloride","Time Factors","Transcription Factors","Transcription, Genetic","Cytochrome P-450 CYP11B2","Mice","Nuclear Receptor Subfamily 4, Group A, Member 2"],"keywords":["Aldosterone","Aldosterone synthase","Cholesterol side-chain cleavage enzyme","Zona glomerulosa","Internal medicine","Endocrinology","Downregulation and upregulation","Steroidogenic acute regulatory protein","Angiotensin II","Renin–angiotensin system","Stimulation","Adrenal cortex","Steroid 11-beta-hydroxylase","Mineralocorticoid","Chemistry","Biology","Gene expression","Medicine","Steroid","Gene","Cytochrome P450","Metabolism","Biochemistry","Hormone","Blood pressure"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-19T15:09:56.545899Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}