{"doi":"10.1667/rade-20-00246.1","title":"Tomotherapy Applied Total Lymphoid Irradiation and Allogeneic Hematopoietic Cell Transplantation Generates Mixed Chimerism in the Rhesus Macaque Model","abstract":"Development of a new methodology to induce immunological chimerism after allogeneic hematopoietic cell (HC) transplantation in a rhesus macaque model is described. The chimeric state was achieved using a non-myeloablative, helical tomotherapy-based total lymphoid irradiation (TomoTLI) conditioning regimen followed by donor HC infusions between 1-haplotype matched donor/recipient pairs. The technique was tested as a feasibility study in an experimental group of seven rhesus macaques that received the novel TomoTLI tolerance protocol and HC allo-transplants. Two tomotherapy protocols were compared: TomoTLI (n = 5) and TomoTLI/total-body irradiation (TBI) (n = 2). Five of seven animals developed mixed chimerism. Three of five animals given the TomoTLI protocol generated transient mixed chimerism with no graft-versus-host disease (GVHD) with survival of 33, 152 and >180 days. However, the inclusion of belatacept in addition to a single fraction of TBI resulted in total chimerism and fatal GVHD in both animals, indicating an unacceptable conditioning regimen.","journal":"Radiation Research","year":2021,"id":202676,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9556,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":783298,"name":"John Fechner","orcid":null,"position":1,"is_corresponding":false},{"id":783299,"name":"Jennifer Post","orcid":null,"position":2,"is_corresponding":false},{"id":783300,"name":"Nathaniel Van Asselt","orcid":null,"position":3,"is_corresponding":false},{"id":783301,"name":"Kevin Kvasnica","orcid":null,"position":4,"is_corresponding":false},{"id":783302,"name":"Lynn D. Haynes","orcid":null,"position":5,"is_corresponding":false},{"id":783303,"name":"Jenny Coonen","orcid":null,"position":6,"is_corresponding":false},{"id":484966,"name":"Kevin Brunner","orcid":"0000-0003-2620-2666","position":7,"is_corresponding":false},{"id":783304,"name":"W. John Haynes","orcid":null,"position":8,"is_corresponding":false},{"id":783305,"name":"Christopher Little","orcid":null,"position":9,"is_corresponding":false},{"id":255412,"name":"William J. Burlingham","orcid":"0000-0002-2653-6044","position":10,"is_corresponding":false},{"id":277447,"name":"Peiman Hematti","orcid":"0000-0001-5836-4497","position":11,"is_corresponding":false},{"id":259286,"name":"Samuel Strober","orcid":"0000-0002-1765-2326","position":12,"is_corresponding":false},{"id":351265,"name":"Dixon B. Kaufman","orcid":"0000-0003-3615-0994","position":13,"is_corresponding":false},{"id":783297,"name":"Lisa Forrest","orcid":null,"position":0,"is_corresponding":true}],"reference_count":30,"raw_metadata":null,"created_at":"2026-07-18T23:51:10.291262Z","pmid":"34388816","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}