{"doi":"10.1634/stemcells.2004-0279","title":"Activin A Maintains Pluripotency of Human Embryonic Stem Cells in the Absence of Feeder Layers","abstract":null,"journal":"STEM CELLS","year":2005,"id":612256,"datarank":15.017806147595632,"base_score":6.218600119691729,"endowment":6.218600119691729,"self_citation_contribution":0.9327900179537595,"citation_network_contribution":14.085016129641872,"self_endowment_contribution":0.9327900179537595,"citer_contribution":14.085016129641872,"corpus_percentile":null,"corpus_rank":null,"citation_count":501,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1576317,"name":"Ana D. Lopez","orcid":null,"position":1,"is_corresponding":false},{"id":1576318,"name":"Nathan Bucay","orcid":null,"position":2,"is_corresponding":false},{"id":1576319,"name":"Andrew Hinton","orcid":null,"position":3,"is_corresponding":false},{"id":1097536,"name":"Meri T. Firpo","orcid":null,"position":4,"is_corresponding":false},{"id":79814,"name":"Charles C. King","orcid":null,"position":5,"is_corresponding":false},{"id":1504595,"name":"Alberto Hayek","orcid":null,"position":6,"is_corresponding":false},{"id":1576316,"name":"Gillian M. Beattie","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Activin A Maintains Pluripotency of Human Embryonic Stem Cells in the Absence of Feeder Layers","abstract":"To date, all human embryonic stem cells (hESCs) available for research require unidentified soluble factors secreted from feeder layers to maintain the undifferentiated state and pluripotency. Activation of STAT3 by leukemia inhibitory factor is required to maintain \"stemness\" in mouse embryonic stem cells, but not in hESCs, suggesting the existence of alternate signaling pathways for self-renewal and pluripotency in human cells. Here we show that activin A is secreted by mouse embryonic feeder layers (mEFs) and that culture medium enriched with activin A is capable of maintaining hESCs in the undifferentiated state for >20 passages without the need for feeder layers, conditioned medium from mEFs, or STAT3 activation. hESCs retained both normal karyotype and markers of undifferentiated cells, including Oct-4, nanog, and TRA-1-60 and remained pluripotent, as shown by the in vivo formation of teratomas.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"15790770","pmcid":null,"openalex_id":null,"authors":[],"funders":[{"funder_name":"NHLBI NIH HHS","grant_id":"T32 HL 07276","title":null}],"total_grants":1,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"bronze","license":"http://doi.wiley.com/10.1002/tdm_license_1.1","oa_locations":[{"url":"https://academic.oup.com/stmcls/article-pdf/23/4/489/41029274/stmcls_23_4_489.pdf","host_type":"publisher"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1634%2Fstemcells.2004-0279","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1634/stemcells.2004-0279","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/full-xml/10.1634/stemcells.2004-0279","host_type":"publisher"}],"fields_of_study":[],"mesh_terms":["Cells, Cultured","Pluripotent Stem Cells","Animals","Humans","Mice","Teratoma","Niacinamide","Activins","Inhibin-beta Subunits","Glycoproteins","Proteoglycans","DNA-Binding Proteins","Homeodomain Proteins","Laminin","Antigens, Surface","Culture Media","Cell Culture Techniques","Karyotyping","Cell Differentiation","Octamer Transcription Factor-3","Fibroblast Growth Factor 7","Embryo, Mammalian","Biomarkers","Nanog Homeobox Protein"],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T02:32:28.427708Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}