{"doi":"10.15829/1728-8800-2025-4550","title":"Effect of long-term plasma storage on the profile of circulating microRNAs","abstract":"<jats:p>\n                    <jats:bold>Aim</jats:bold>\n                    . To assess the impact of long-term storage of biobanked plasma samples on the profile of circulating small non-coding ribonucleic acids (microRNAs).\n                  </jats:p>\n                  <jats:p>\n                    <jats:bold>Material and methods</jats:bold>\n                    . The study included paired plasma aliquots from 10 patients from the biobank collection of the National Medical Research Center for Therapy and Preventive Medicine. The control group consisted of microRNA samples isolated 1,5 years after plasma collection and then stored in aqueous solution for 3,5 years. In the long-term plasma storage group, microRNA was isolated from a second plasma aliquot after 5 years. All samples were stored at -70 оC. Se­quen­cing was performed for both groups simultaneously on the NextSeq 550 platform (Illumina, USA) using the High Output 1×75 bp protocol.\n                  </jats:p>\n                  <jats:p>\n                    <jats:bold>Results.</jats:bold>\n                    Principal component analysis based on human microRNA gene expression data (ENCODE v47) revealed heterogeneity between the study groups. In the long-term plasma storage group, compared to the control group, a significant decrease in library concentration and size was observed, as well as a more than twofold increase in expression levels for 31 microRNAs.\n                  </jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion.</jats:bold>\n                    Circulating microRNAs demonstrated higher stability du­ring storage in plasma than in aqueous solution. The obtained results in­di­cate the need to consider the storage time of isolated microRNA, along with other preanalytical factors, to improve the reproducibility of micro­RNA studies.\n                  </jats:p>","journal":"Cardiovascular Therapy and Prevention","year":2026,"id":3871,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":0.0,"corpus_rank":765,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":true,"is_dataset_confidence":0.5502,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2026-01-23","fair_score":22.9167,"fair_percentile":2.484608619173263,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":39625,"name":"E. A. Sotnikova","orcid":"0000-0002-8395-4146","position":1,"is_corresponding":false},{"id":39626,"name":"A. A. Zharikova","orcid":"0000-0003-0723-0493","position":2,"is_corresponding":false},{"id":39627,"name":"V. A. Kutsenko","orcid":"0000-0001-9844-3122","position":3,"is_corresponding":false},{"id":39628,"name":"D. K. Vasiliev","orcid":"0000-0003-2602-5006","position":4,"is_corresponding":false},{"id":39629,"name":"M. S. Pokrovskaya","orcid":"0000-0001-6985-7131","position":5,"is_corresponding":false},{"id":39630,"name":"A. I. Ershova","orcid":"0000-0001-7989-0760","position":6,"is_corresponding":false},{"id":39631,"name":"A. N. Meshkov","orcid":"0000-0001-5989-6233","position":7,"is_corresponding":false},{"id":39632,"name":"O. M. Drapkina","orcid":"0000-0002-4453-8430","position":8,"is_corresponding":false},{"id":39624,"name":"A. V. Kiseleva","orcid":"0000-0003-4765-8021","position":0,"is_corresponding":true}],"reference_count":28,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":20.0,"fair_a":30.0,"fair_i":25.0,"fair_r":16.6667,"fair_zscore":-2.0167,"fair_rationale":{"fair_score":22.92,"has_llm":true,"dimensions":{"F":{"name":"Findable","score":20.0,"criteria":[{"key":"f_has_doi","label":"Has a persistent DOI","kind":"deterministic","weight":1.0,"fraction":1.0,"signal":"DOI present","rationale":null},{"key":"f_repository_presence","label":"Indexed in repositories / literature DBs","kind":"deterministic","weight":1.0,"fraction":0.0,"signal":"datacite=0, pmcid=False, pmid=False","rationale":null},{"key":"f_persistent_ids","label":"Resolvable scholarly identifiers (OpenAlex)","kind":"deterministic","weight":0.5,"fraction":0.0,"signal":"no OpenAlex id","rationale":null},{"key":"f_metadata_richness","label":"Rich, machine-readable metadata","kind":"llm","weight":1.0,"fraction":0.0,"signal":null,"rationale":"The paper provides no machine-readable or structured metadata (e.g., DCAT, schema.org) and only contains human-readable prose describing sample conditions and sequencing parameters."}]},"A":{"name":"Accessible","score":30.0,"criteria":[{"key":"a_open_access","label":"Open Access / files deposited","kind":"deterministic","weight":1.5,"fraction":1.0,"signal":"Open Access","rationale":null},{"key":"a_retrievable","label":"Free full text retrievable","kind":"deterministic","weight":1.0,"fraction":0.0,"signal":"0 OA location(s)","rationale":null},{"key":"a_access_protocol","label":"Clear data/code access protocol","kind":"llm","weight":1.0,"fraction":0.0,"signal":null,"rationale":"No data or code access protocol is stated; the text does not mention any repository, data availability statement, or method to retrieve raw or processed files."}]},"I":{"name":"Interoperable","score":25.0,"criteria":[{"key":"i_linked_data","label":"Linked datasets / DataCite relations","kind":"deterministic","weight":1.0,"fraction":0.0,"signal":"linked_datasets=0, datacite=0","rationale":null},{"key":"i_standard_ids","label":"References data via standard accessions","kind":"deterministic","weight":1.0,"fraction":0.0,"signal":"accessions=0, trials=0","rationale":null},{"key":"i_standards","label":"Standard formats, vocabularies & identifiers","kind":"llm","weight":1.0,"fraction":0.5,"signal":null,"rationale":"Standard formats (fastq, GRCh38 genome, ENCODE v47 annotation) and platform-specific identifiers (Illumina, Qiagen kits) are used, but no persistent identifiers (e.g., DOIs for datasets) or controlled vocabulary are provided."}]},"R":{"name":"Reusable","score":16.67,"criteria":[{"key":"r_license","label":"Clear, open reuse license","kind":"deterministic","weight":1.5,"fraction":0.0,"signal":"no license","rationale":null},{"key":"r_downloads","label":"Demonstrated reuse (downloads)","kind":"deterministic","weight":0.5,"fraction":0.0,"signal":"downloads=0","rationale":null},{"key":"r_version","label":"Versioned / maintained","kind":"deterministic","weight":0.5,"fraction":0.0,"signal":"no version chain","rationale":null},{"key":"r_dataset","label":"Classified as a data resource","kind":"deterministic","weight":0.5,"fraction":1.0,"signal":"is_dataset","rationale":null},{"key":"r_reusability","label":"Data-availability statement, license & reproducibility","kind":"llm","weight":2.0,"fraction":0.167,"signal":null,"rationale":"No data-availability statement, license, or public repository link is provided, and only minimal methodological details (e.g., kit versions, software names) are given without sharing scripts or raw data, severely limiting reproducibility."}]}},"suggestions":["Add a data-availability statement with a DOI or persistent link to a public repository (e.g., GEO, ArrayExpress) containing raw sequencing data and processed counts.","Provide machine-readable metadata (e.g., as an ISA-Tab or JSON-LD file) describing sample storage durations, isolation protocols, and sequencing parameters.","Include a software/code availability statement and deposit analysis scripts (e.g., for DESeq2, PCA) in a repository like GitHub or Zenodo with a license.","Specify a clear protocol for accessing the biobank samples (e.g., contact information and material transfer agreement conditions) to enable validation.","Add a license (e.g., CC-BY) to the paper explicitly to clarify reuse permissions and cite a data usage policy."],"model":"deepseek/deepseek-v4-flash","agent_version":"fair_agent_v2","fulltext_source":"unpaywall_pdf"},"fair_model":"deepseek/deepseek-v4-flash","fair_agent_version":"fair_agent_v2","fair_fulltext_source":"unpaywall_pdf","fair_has_llm":true,"fair_computed_at":"2026-06-18T06:45:22.140204Z","clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}