{"doi":"10.1538/expanim.55.57","title":"Comparison of K-Cl Cotransport Expression in High and Low K Dog Erythrocytes","abstract":null,"journal":"Experimental Animals","year":2006,"id":600874,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1540552,"name":"Kazunari HIGA","orcid":null,"position":1,"is_corresponding":false},{"id":1540553,"name":"Shin HISAMATSU","orcid":null,"position":2,"is_corresponding":false},{"id":1540554,"name":"Hiroshi FUJISE","orcid":null,"position":3,"is_corresponding":false},{"id":1540551,"name":"Hideharu OCHIAI","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Comparison of K-Cl Cotransport Expression in High and Low K Dog Erythrocytes","abstract":"K-Cl cotransport plays a crucial role in regulatory volume decrease of erythrocytes. K-Cl cotransport activities in dog erythrocytes with an inherited high Na-K pump activity (HK) and normal erythrocytes (LK) were compared. Nitrite (NO(2)) stimulated K-Cl cotransport activity in HK cells around 14-fold at 2.4 mM, and it also increased the Km value of this cotransporter. Real-time PCR and western blot analysis revealed that K-Cl cotransporter 1 was dominant, and that the quantity of K-Cl cotransporter 1 protein was comparable between HK and LK erythrocytes. These results suggest that the difference in cotransport activity was not caused by the amount of K-Cl cotransport protein but by a difference in the regulation system, which is susceptible to oxidant.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16508213","pmcid":null,"openalex_id":"https://openalex.org/W2051086962","authors":[],"funders":[],"total_grants":0,"fwci":0.0,"citation_percentile":0.13548925,"influential_citations":0,"citation_trend":[],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://www.jstage.jst.go.jp/article/expanim/55/1/55_1_57/_pdf","host_type":"journal"},{"url":"https://www.jstage.jst.go.jp/article/expanim/55/1/55_1_57/_pdf","host_type":"publisher"},{"url":"http://www.jstage.jst.go.jp/article/expanim/55/1/55_1_57/_pdf","host_type":"publisher"},{"url":"https://doi.org/10.1538/expanim.55.57","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/16508213","host_type":"repository"}],"fields_of_study":["Erythrocyte Function and Pathophysiology","Ion Transport and Channel Regulation","Ion channel regulation and function","Animals","Cell Size","Chlorides","Dogs","Dose-Response Relationship, Drug","Erythrocytes","Ouabain","Potassium","Reverse Transcriptase Polymerase Chain Reaction","Rubidium","Sodium Nitrite","Symporters","K Cl- Cotransporters"],"mesh_terms":["K Cl- Cotransporters","Animals","Chlorides","Dogs","Dose-Response Relationship, Drug","Erythrocytes","Ouabain","Potassium","Rubidium","Sodium Nitrite","Reverse Transcriptase Polymerase Chain Reaction","Symporters","Cell Size"],"keywords":["Cotransporter","Western blot","Chemistry","Red blood cell","Biochemistry","Molecular biology","Biology","Gene","Sodium"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T14:23:16.338900Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}