{"doi":"10.1534/genetics.120.303052","title":"The Dynamics of P Granule Liquid Droplets Are Regulated by the<i>Caenorhabditis elegans</i>Germline RNA Helicase GLH-1 via Its ATP Hydrolysis Cycle","abstract":"Abstract Germ granules, also known as P granules in Caenorhabditis elegans, are phase-separated cellular bodies that are frequently found at the perinuclear region of germ cell nuclei in various animals. However... P granules are phase-separated liquid droplets that play important roles in the maintenance of germ cell fate in Caenorhabditis elegans. Both the localization and formation of P granules are highly dynamic, but mechanisms that regulate such processes remain poorly understood. Here, we show evidence that the VASA-like germline RNA helicase GLH-1 couples distinct steps of its ATPase hydrolysis cycle to control the formation and disassembly of P granules. In addition, we found that the phenylalanine-glycine-glycine repeats in GLH-1 promote its localization at the perinucleus. Proteomic analyses of the GLH-1 complex with a GLH-1 mutation that interferes with P granule disassembly revealed transient interactions of GLH-1 with several Argonautes and RNA-binding proteins. Finally, we found that defects in recruiting the P granule component PRG-1 to perinuclear foci in the adult germline correlate with the fertility defects observed in various GLH-1 mutants. Together, our results highlight the versatile roles of an RNA helicase in controlling the formation of liquid droplets in space and time.","journal":"Genetics","year":2020,"id":92783,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":41,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9578,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":464680,"name":"Yabing Hu","orcid":null,"position":1,"is_corresponding":false},{"id":463803,"name":"Charles F. Lang","orcid":"0000-0001-6428-3941","position":2,"is_corresponding":false},{"id":463804,"name":"Jordan Brown","orcid":"0000-0001-9880-4784","position":3,"is_corresponding":false},{"id":464681,"name":"Sierra Schwabach","orcid":null,"position":4,"is_corresponding":false},{"id":463805,"name":"Xiao‐Yan Song","orcid":"0000-0002-6396-964X","position":5,"is_corresponding":false},{"id":463806,"name":"Ying Zhang","orcid":"0000-0002-2018-7728","position":6,"is_corresponding":false},{"id":309231,"name":"Edwin Munro","orcid":"0000-0001-5535-5675","position":7,"is_corresponding":false},{"id":463807,"name":"Karen L. Bennett","orcid":"0000-0002-6052-379X","position":8,"is_corresponding":false},{"id":463808,"name":"Donglei Zhang","orcid":"0000-0002-2720-1414","position":9,"is_corresponding":false},{"id":463809,"name":"Heng-Chi Lee","orcid":"0000-0001-8154-906X","position":10,"is_corresponding":false},{"id":463802,"name":"Wenjun Chen","orcid":"0000-0002-2458-7040","position":0,"is_corresponding":true}],"reference_count":48,"raw_metadata":null,"created_at":"2026-07-18T22:30:31.563852Z","pmid":"32245789","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}