{"doi":"10.1534/genetics.112.147421","title":"The Mechanism of Nucleotide Excision Repair-Mediated UV-Induced Mutagenesis in Nonproliferating Cells","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>Following the irradiation of nondividing yeast cells with ultraviolet (UV) light, most induced mutations are inherited by both daughter cells, indicating that complementary changes are introduced into both strands of duplex DNA prior to replication. Early analyses demonstrated that such two-strand mutations depend on functional nucleotide excision repair (NER), but the molecular mechanism of this unique type of mutagenesis has not been further explored. In the experiments reported here, an ade2 adeX colony-color system was used to examine the genetic control of UV-induced mutagenesis in nondividing cultures of Saccharomyces cerevisiae. We confirmed a strong suppression of two-strand mutagenesis in NER-deficient backgrounds and demonstrated that neither mismatch repair nor interstrand crosslink repair affects the production of these mutations. By contrast, proteins involved in the error-prone bypass of DNA damage (Rev3, Rev1, PCNA, Rad18, Pol32, and Rad5) and in the early steps of the DNA-damage checkpoint response (Rad17, Mec3, Ddc1, Mec1, and Rad9) were required for the production of two-strand mutations. There was no involvement, however, for the Pol η translesion synthesis DNA polymerase, the Mms2-Ubc13 postreplication repair complex, downstream DNA-damage checkpoint factors (Rad53, Chk1, and Dun1), or the Exo1 exonuclease. Our data support models in which UV-induced mutagenesis in nondividing cells occurs during the Pol ζ-dependent filling of lesion-containing, NER-generated gaps. The requirement for specific DNA-damage checkpoint proteins suggests roles in recruiting and/or activating factors required to fill such gaps.</jats:p>","journal":"Genetics","year":2013,"id":36400,"datarank":1.1791135522586806,"base_score":3.4965075614664802,"endowment":3.4965075614664802,"self_citation_contribution":0.5244761342199721,"citation_network_contribution":0.6546374180387085,"self_endowment_contribution":0.5244761342199721,"citer_contribution":0.6546374180387085,"corpus_percentile":null,"corpus_rank":null,"citation_count":32,"citer_count":30,"citers_with_citation_signal":24,"citers_with_endowment":24,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":4638,"name":"Sue Jinks-Robertson","orcid":"0000-0001-5924-5852","position":1,"is_corresponding":false},{"id":184410,"name":"Stanislav G Kozmin","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.4965075614664802,"endowment":3.4965075614664802,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23307894","pmcid":"PMC3583999","openalex_id":"https://openalex.org/W2104129156","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM064769","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"3R01-GM-064769-09S1","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"5R01-GM064769-09","title":null}],"total_grants":3,"fwci":1.7946,"citation_percentile":0.84970703,"influential_citations":2,"citation_trend":[{"year":2013,"count":1},{"year":2014,"count":1},{"year":2015,"count":6},{"year":2016,"count":4},{"year":2017,"count":2},{"year":2018,"count":2},{"year":2020,"count":2},{"year":2021,"count":3},{"year":2022,"count":1},{"year":2023,"count":3},{"year":2024,"count":3},{"year":2025,"count":4}],"oa_status":"bronze","license":"https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model","oa_locations":[{"url":"https://www.genetics.org/content/genetics/193/3/803.full.pdf","host_type":"journal"},{"url":"https://www.genetics.org/content/genetics/193/3/803.full.pdf","host_type":"BRONZE"},{"url":"https://www.genetics.org/content/genetics/193/3/803.full.pdf","host_type":"publisher"},{"url":"https://academic.oup.com/genetics/article-pdf/193/3/803/42119935/genetics0803.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1534/genetics.112.147421","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/23307894","host_type":"repository"},{"url":"http://europepmc.org/articles/PMC3583999","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3583999","host_type":"repository"}],"fields_of_study":["DNA Repair Mechanisms","Carcinogens and Genotoxicity Assessment","CRISPR and Genetic Engineering","Biology","Medicine","Cell Cycle Checkpoints","Cell Cycle Proteins","Cell Division","DNA Damage","DNA Mismatch Repair","DNA Repair","DNA-Directed DNA Polymerase","Mutagenesis","Mutation Rate","Saccharomyces cerevisiae","Saccharomyces cerevisiae Proteins","Ultraviolet Rays"],"mesh_terms":["Cell Division","DNA Damage","DNA-Directed DNA Polymerase","DNA Repair","Saccharomyces cerevisiae","Ultraviolet Rays","Mutagenesis","Cell Cycle Proteins","Saccharomyces cerevisiae Proteins","DNA Mismatch Repair","Cell Cycle Checkpoints","Mutation Rate"],"keywords":["Biology","Mutagenesis","Postreplication repair","Nucleotide excision repair","DNA damage","Pyrimidine dimer","DNA repair","Mutation","DNA","DNA polymerase","Genetics","DNA replication","Saccharomyces cerevisiae","Molecular biology","Cell biology","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-10T15:52:28.990437Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}