{"doi":"10.1534/genetics.108.087189","title":"Mechanisms of Rad52-Independent Spontaneous and UV-Induced Mitotic Recombination in<i>Saccharomyces cerevisiae</i>","abstract":"<jats:title>Abstract</jats:title><jats:p>In wild-type diploid cells, heteroallelic recombination between his4A and his4C alleles leads mostly to His+ gene conversions that have a parental configuration of flanking markers, but ∼22% of recombinants have associated reciprocal crossovers. In rad52 strains, gene conversion is reduced 75-fold and the majority of His+ recombinants are crossover associated, with the largest class being half-crossovers in which the other participating chromatid is lost. We report that UV irradiating rad52 cells results in an increase in overall recombination frequency, comparable to increases induced in wild-type (WT) cells, and surprisingly results in a pattern of recombination products quite similar to RAD52 cells: gene conversion without exchange is favored, and the number of 2n − 1 events is markedly reduced. Both spontaneous and UV-induced RAD52-independent recombination depends strongly on Rad50, whereas rad50 has no effect in cells restored to RAD52. The high level of noncrossover gene conversion outcomes in UV-induced rad52 cells depends on Rad51, but not on Rad59. Those outcomes also rely on the UV-inducible kinase Dun1 and Dun1's target, the repressor Crt1, whereas gene conversion events arising spontaneously depend on Rad59 and Crt1. Thus, there are at least two Rad52-independent recombination pathways in budding yeast.</jats:p>","journal":"Genetics","year":2008,"id":16263,"datarank":1.9084774249115135,"base_score":3.784189633918261,"endowment":3.784189633918261,"self_citation_contribution":0.5676284450877392,"citation_network_contribution":1.3408489798237742,"self_endowment_contribution":0.5676284450877392,"citer_contribution":1.3408489798237742,"corpus_percentile":null,"corpus_rank":null,"citation_count":43,"citer_count":42,"citers_with_citation_signal":37,"citers_with_endowment":37,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":120693,"name":"Taya Feldman","orcid":null,"position":1,"is_corresponding":false},{"id":120694,"name":"Allison S Landman","orcid":null,"position":2,"is_corresponding":false},{"id":120695,"name":"James E Haber","orcid":null,"position":3,"is_corresponding":false},{"id":120692,"name":"Eric Coïc","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.784189633918261,"endowment":3.784189633918261,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18458103","pmcid":"PMC2390599","openalex_id":"https://openalex.org/W2135246088","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM20056","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM020056","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R37 GM020056","title":null}],"total_grants":3,"fwci":1.8248,"citation_percentile":0.84911966,"influential_citations":3,"citation_trend":[{"year":2012,"count":2},{"year":2013,"count":2},{"year":2014,"count":5},{"year":2015,"count":1},{"year":2016,"count":2},{"year":2017,"count":4},{"year":2018,"count":4},{"year":2019,"count":2},{"year":2020,"count":3},{"year":2021,"count":2},{"year":2022,"count":1},{"year":2023,"count":2}],"oa_status":"bronze","license":"https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model","oa_locations":[{"url":"https://academic.oup.com/genetics/article-pdf/179/1/199/49412488/genetics0199.pdf","host_type":"journal"},{"url":"https://academic.oup.com/genetics/article-pdf/179/1/199/49412488/genetics0199.pdf","host_type":"BRONZE"},{"url":"https://academic.oup.com/genetics/article-pdf/179/1/199/49412488/genetics0199.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1534/genetics.108.087189","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/18458103","host_type":"repository"},{"url":"https://hal.science/hal-04278553","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2390599","host_type":"repository"}],"fields_of_study":["DNA Repair Mechanisms","CRISPR and Genetic Engineering","Photosynthetic Processes and Mechanisms","Biology","Medicine","Mitosis","Rad52 DNA Repair and Recombination Protein","Recombination, Genetic","Saccharomyces cerevisiae","Saccharomyces cerevisiae Proteins","Ultraviolet Rays"],"mesh_terms":["Mitosis","Recombination, Genetic","Saccharomyces cerevisiae","Ultraviolet Rays","Saccharomyces cerevisiae Proteins","Rad52 DNA Repair and Recombination Protein"],"keywords":["Mitotic crossover","RAD52","Gene conversion","Biology","Saccharomyces cerevisiae","RAD51","Genetics","Homologous recombination","FLP-FRT recombination","Recombination","Chromosomal crossover","Ectopic recombination","Gene","Chromatid","Genetic recombination","Allele","Molecular biology","Chromosome"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-01T21:05:55.062161Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}