{"doi":"10.1534/g3.120.401654","title":"The Beginning of the End: A Chromosomal Assembly of the New World Malaria Mosquito Ends with a Novel Telomere","abstract":"Abstract Chromosome level assemblies are accumulating in various taxonomic groups including mosquitoes. However, even in the few reference-quality mosquito assemblies, a significant portion of the heterochromatic regions including telomeres remain unresolved. Here we produce a de novo assembly of the New World malaria mosquito, Anopheles albimanus by integrating Oxford Nanopore sequencing, Illumina, Hi-C and optical mapping. This 172.6 Mbps female assembly, which we call AalbS3, is obtained by scaffolding polished large contigs (contig N50 = 13.7 Mbps) into three chromosomes. All chromosome arms end with telomeric repeats, which is the first in mosquito assemblies and represents a significant step toward the completion of a genome assembly. These telomeres consist of tandem repeats of a novel 30-32 bp Telomeric Repeat Unit (TRU) and are confirmed by analyzing the termini of long reads and through both chromosomal in situ hybridization and a Bal31 sensitivity assay. The AalbS3 assembly included previously uncharacterized centromeric and rDNA clusters and more than doubled the content of transposable elements and other repetitive sequences. This telomere-to-telomere assembly, although still containing gaps, represents a significant step toward resolving biologically important but previously hidden genomic components. The comparison of different scaffolding methods will also inform future efforts to obtain reference-quality genomes for other mosquito species.","journal":"G3 Genes Genomes Genetics","year":2020,"id":99394,"datarank":0.8302393841485501,"base_score":3.4339872044851463,"endowment":3.4339872044851463,"self_citation_contribution":0.515098080672772,"citation_network_contribution":0.3151413034757782,"self_endowment_contribution":0.515098080672772,"citer_contribution":0.3151413034757782,"corpus_percentile":75.70201902993735,"corpus_rank":3142,"citation_count":30,"citer_count":20,"citers_with_citation_signal":17,"citers_with_endowment":17,"datacite_reuse_total":0,"is_dataset":true,"is_dataset_confidence":0.7779,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":489096,"name":"Jiangtao Liang","orcid":"0000-0002-8468-7457","position":1,"is_corresponding":false},{"id":489097,"name":"Chujia Chen","orcid":"0000-0002-9196-1274","position":2,"is_corresponding":false},{"id":489098,"name":"Varvara Lukyanchikova","orcid":"0000-0002-1828-4721","position":3,"is_corresponding":false},{"id":293600,"name":"Yumin Qi","orcid":null,"position":4,"is_corresponding":false},{"id":489099,"name":"Mark Potters","orcid":"0009-0009-9983-0031","position":5,"is_corresponding":false},{"id":302894,"name":"Robert E. Settlage","orcid":"0000-0002-1354-7609","position":6,"is_corresponding":false},{"id":489100,"name":"Dustin B. Miller","orcid":"0000-0001-8387-9791","position":7,"is_corresponding":false},{"id":489101,"name":"Stéphane Deschamps","orcid":"0000-0001-5175-3377","position":8,"is_corresponding":false},{"id":293602,"name":"Chunhong Mao","orcid":null,"position":9,"is_corresponding":false},{"id":489102,"name":"Víctor Llaca","orcid":"0000-0003-4822-2924","position":10,"is_corresponding":false},{"id":50345,"name":"Igor V. Sharakhov","orcid":"0000-0003-0752-3747","position":11,"is_corresponding":false},{"id":262211,"name":"Zhijian Tu","orcid":"0000-0003-4227-3819","position":12,"is_corresponding":false},{"id":489095,"name":"Austin Compton","orcid":"0000-0002-1135-0052","position":0,"is_corresponding":true}],"reference_count":49,"raw_metadata":null,"created_at":"2026-07-18T22:37:35.547042Z","pmid":"32883756","pmcid":"PMC7534423","fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}