{"doi":"10.1534/g3.120.401133","title":"Cas9-Mediated Gene-Editing in the Malaria Mosquito <i>Anopheles stephensi</i> by ReMOT Control","abstract":"Innovative tools are essential for advancing malaria control and depend on an understanding of molecular mechanisms governing transmission of malaria parasites by Anopheles mosquitoes. CRISPR/Cas9-based gene disruption is a powerful method to uncover underlying biology of vector-pathogen interactions and can itself form the basis of mosquito control strategies. However, embryo injection methods used to genetically manipulate mosquitoes (especially Anopheles ) are difficult and inefficient, particularly for non-specialist laboratories. Here, we adapted the ReMOT Control ( Re ceptor- m ediated O vary T ransduction of C argo) technique to deliver Cas9 ribonucleoprotein complex to adult mosquito ovaries, generating targeted and heritable mutations in the malaria vector Anopheles stephensi without injecting embryos. In Anopheles , ReMOT Control gene editing was as efficient as standard embryo injections. The application of ReMOT Control to Anopheles opens the power of CRISPR/Cas9 methods to malaria laboratories that lack the equipment or expertise to perform embryo injections and establishes the flexibility of ReMOT Control for diverse mosquito species.","journal":"G3 Genes Genomes Genetics","year":2020,"id":56290,"datarank":0.6698862177981877,"base_score":4.465908118654584,"endowment":4.465908118654584,"self_citation_contribution":0.6698862177981877,"citation_network_contribution":0.0,"self_endowment_contribution":0.6698862177981877,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":86,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9512,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":293698,"name":"Sage McKeand","orcid":null,"position":1,"is_corresponding":false},{"id":292320,"name":"Duverney Chaverra‐Rodriguez","orcid":"0000-0001-8068-7436","position":2,"is_corresponding":false},{"id":230622,"name":"Grant L. Hughes","orcid":"0000-0002-7567-7185","position":3,"is_corresponding":false},{"id":293699,"name":"Aniko Fazekas","orcid":null,"position":4,"is_corresponding":false},{"id":292321,"name":"Sujit Pujhari","orcid":"0000-0001-5856-5328","position":5,"is_corresponding":false},{"id":245842,"name":"Nijole Jasinskiene","orcid":"0000-0002-0862-0349","position":6,"is_corresponding":false},{"id":245852,"name":"Anthony A. James","orcid":"0000-0001-5577-3308","position":7,"is_corresponding":false},{"id":275640,"name":"Jason L. Rasgon","orcid":"0000-0002-4050-8429","position":8,"is_corresponding":false},{"id":292319,"name":"Vanessa M. Macias","orcid":"0000-0002-8008-7403","position":0,"is_corresponding":true}],"reference_count":18,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T21:05:49.581291Z","pmid":"32122959","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}