{"doi":"10.1530/eje.0.1500235","title":"Effect of GHRH and GHRP-2 treatment in vitro on GH secretion and levels of GH, pituitary transcription factor-1, GHRH-receptor, GH-secretagogue-receptor and somatostatin receptor mRNAs in ovine pituitary cells","abstract":"<jats:p>OBJECTIVE: Growth hormone (GH)-releasing hormone (GHRH) and GH-releasing peptides (GHRPs) stimulate the release of GH through their specific receptors on somatotropes. Combined GHRH and GHRP administration causes a synergistic GH release in vivo by an unknown mechanism. The current study focuses on the direct action of GHRH and GHRP on several molecular targets in somatotropes. DESIGN AND METHODS: To clarify the mechanism of action, ovine somatotropes were used to measure the expression of mRNAs encoding for GH, pituitary transcription factor-1 (Pit-1), GH-secretagogue receptor (GHS-R), GHRH-R, somatostatin receptor subtypes (sst-1 and sst-2) and GH release after GHRH and GHRP-2 treatment for 0.5, 1, 1.5 and 2 h. RESULTS: GHRH (10 nM), GHRP-2 (100 nM) and combined GHRH-GHRP-2 increased the levels of GH mRNA and GH release from 0.5 to 2 h in a time-dependent manner. The levels of Pit-1, GHRH-R and GHS-R mRNA were increased after 0.5 h treatment of cells with GHRH and GHRP-2. The levels of sst-1 but not sst-2 mRNA were significantly increased after 0.5 and 1 h of GHRH treatment. In contrast, both sst-1 and sst-2 mRNA expression was inhibited after 0.5-2 h of GHRP treatment. CONCLUSIONS: These data demonstrate a direct in vitro modification of ovine somatotropes by GHRH and GHRP-2 resulting in altered GHRH-R, GHS-R, Pit-1, sst-1, sst-2 and GH gene expression; this may underlie the regulatory action of GHRH and GHRP-2 on GH secretion.</jats:p>","journal":"European Journal of Endocrinology","year":2004,"id":15460,"datarank":0.9967208476842999,"base_score":3.044522437723423,"endowment":3.044522437723423,"self_citation_contribution":0.4566783656585135,"citation_network_contribution":0.5400424820257864,"self_endowment_contribution":0.4566783656585135,"citer_contribution":0.5400424820257864,"corpus_percentile":null,"corpus_rank":null,"citation_count":20,"citer_count":17,"citers_with_citation_signal":15,"citers_with_endowment":15,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":117861,"name":"M Hernandez","orcid":null,"position":1,"is_corresponding":false},{"id":117862,"name":"R Xu","orcid":null,"position":2,"is_corresponding":false},{"id":117863,"name":"C Chen","orcid":null,"position":3,"is_corresponding":false},{"id":117860,"name":"M Yan","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.044522437723423,"endowment":3.044522437723423,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"14763922","pmcid":null,"openalex_id":"https://openalex.org/W2102574055","authors":[],"funders":[],"total_grants":0,"fwci":2.013,"citation_percentile":0.87226573,"influential_citations":0,"citation_trend":[{"year":2014,"count":2},{"year":2024,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://eje.bioscientifica.com/downloadpdf/journals/eje/150/2/235.pdf","host_type":"BRONZE"},{"url":"https://eje.bioscientifica.com/view/journals/eje/150/2/235.xml","host_type":"publisher"},{"url":"https://doi.org/10.1530/eje.0.1500235","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/14763922","host_type":"repository"}],"fields_of_study":["Growth Hormone and Insulin-like Growth Factors","Stress Responses and Cortisol","Hypothalamic control of reproductive hormones","Medicine","Chemistry","Animals","Cells, Cultured","DNA-Binding Proteins","Gene Expression Regulation","Growth Hormone","Growth Hormone-Releasing Hormone","Oligopeptides","Pituitary Gland","RNA, Messenger","Receptors, Cell Surface","Receptors, G-Protein-Coupled","Receptors, Ghrelin","Receptors, Neuropeptide","Receptors, Pituitary Hormone-Regulating Hormone","Receptors, Somatostatin","Sheep","Transcription Factor Pit-1","Transcription Factors"],"mesh_terms":["Animals","Cells, Cultured","DNA-Binding Proteins","Gene Expression Regulation","Oligopeptides","Pituitary Gland","Receptors, Cell Surface","RNA, Messenger","Sheep","Growth Hormone","Growth Hormone-Releasing Hormone","Transcription Factors","Receptors, Somatostatin","Receptors, Neuropeptide","Receptors, Pituitary Hormone-Regulating Hormone","Receptors, G-Protein-Coupled","Transcription Factor Pit-1","Receptors, Ghrelin"],"keywords":["Internal medicine","Endocrinology","Somatotropic cell","Secretagogue","Somatostatin","Growth hormone–releasing hormone","Somatostatin receptor 2","Biology","Receptor","Pituitary gland","Chemistry","Hormone","Somatostatin receptor","Stimulation","Medicine","Growth hormone"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-01T17:49:25.275880Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}