{"doi":"10.1530/acta.0.0760629","title":"BIOASSAY OF ANTIGONADOTROPHIC SERA","abstract":"<jats:title>ABSTRACT</jats:title>\n        <jats:p>Data are presented on the reliability of the previously described bioassay of gonadotrophin neutralizing potency of antisera raised against HCG preparations. The <jats:italic>sensitivity</jats:italic> of the method may be defined as the smallest detectable neutralizing activity expressed in anti-units (AU). This depends partly on the bioassay system used, and partly on the intended percentage of neutralization. In the HCG bioassay used in the present study, and around 50 % neutralization, the smallest detectable neutralizing activity is about 1.0 AU. The sensitivity decreases with increasing levels of neutralization: it is about 9 AU around 90% neutralization. The <jats:italic>precision</jats:italic> as given by the mean index of precision (λ) was 0.12 in 52 assays. The 95 % fiducial limits expressed as a percentage of the mean potency estimate ranged from 65.8 % to 125.7% in 52 assays conducted at 50 % and from 89.1% to 105.3% in 25 assays conducted at 90% intended neutralization. <jats:italic>Reproducibility:</jats:italic> eighteen antisera raised in 18 rabbits against 9 HCG preparations were assayed at 50% intended neutralization repeatedly (at least twice each antiserum) in 52 assays. Only one antiserum showed significant heterogeneity among repeated estimates by a χ2 test. The achieved neutralization ranged from 36 % to 79 % with a mean of 50 %.</jats:p>\n        <jats:p>The neutralizing potency estimates of 19 antisera raised in 19 rabbits against 10 HCG preparations in 45 assays at 50% intended neutralization and in 25 assays at 90 % intended neutralization were also compared. These potency estimates were in close agreement with each other at both levels of neutralization (except for one antiserum) as evidenced by the results of χ<jats:sup>2</jats:sup> test and the value of the correlation coefficient obtained (r = 0.99). It is concluded that \"the principle of simple additivity\" is a satisfactory approximation to represent the antigen - antibody reaction involved in the neutralization of the biological activity of HCG preparations.</jats:p>","journal":"Acta Endocrinologica","year":1974,"id":21825,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":1,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":139135,"name":"C. Robyn","orcid":null,"position":1,"is_corresponding":false},{"id":139136,"name":"P. Petrusz","orcid":null,"position":2,"is_corresponding":false},{"id":139137,"name":"E. Diczfalusy","orcid":null,"position":3,"is_corresponding":false},{"id":139134,"name":"P. Romaní","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21071399","pmcid":null,"openalex_id":"https://openalex.org/W4232026532","authors":[],"funders":[],"total_grants":0,"fwci":0.2196,"citation_percentile":0.55320264,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://eje.bioscientifica.com/view/journals/eje/76/3/acta_76_3_019.xml","host_type":"publisher"},{"url":"https://doi.org/10.1530/acta.0.0760629","host_type":"journal"}],"fields_of_study":["Viral Infectious Diseases and Gene Expression in Insects","Protein purification and stability"],"mesh_terms":[],"keywords":["Neutralization","Potency","Bioassay","Antiserum","Chemistry","Neutralizing antibody","Chromatography","Antibody","Biology","Immunology","In vitro","Biochemistry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-06T16:55:07.953665Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}