{"doi":"10.1530/acta.0.062s257","title":"RADIOIMMUNOASSAY EMPLOYING POLYMERIZED ANTISERA","abstract":"<jats:title>ABSTRACT</jats:title>\n        <jats:p>Antisera made insoluble by polymerization were used for the radioimmunoassay of FSH and LH, as this was shown to be a useful method of separating bound and free labelled hormone.</jats:p>\n        <jats:p>Highly purified HCG and FSH, prepared in this laboratory, from urinary source were labelled with <jats:sup>125</jats:sup>I. Antisera were raised by injecting urinary materials. The specificity of antisera was studied and some cross-reactivity was found between anti-urinary FSH and LH, but not with HCG. The sensitivity of LH and FSH radioimmunoassay was 1 mIU/ml and 3 mIU/ml, respectively.</jats:p>\n        <jats:p>Some determinations in dialyzed urine from various sources have also been performed and the preliminary results obtained are compared to those previously reported by others.</jats:p>\n        <jats:p>This paper discusses the radioimmunoassay of FSH and LH using a new solid-phase method, i. e. the use of polymerized antiserum, as previously described (<jats:italic>Donini et al.</jats:italic> 1968).</jats:p>","journal":"Acta Endocrinologica","year":1969,"id":44782,"datarank":0.8869817076786979,"base_score":2.639057329615259,"endowment":2.639057329615259,"self_citation_contribution":0.3958585994422889,"citation_network_contribution":0.491123108236409,"self_endowment_contribution":0.3958585994422889,"citer_contribution":0.491123108236409,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":13,"citers_with_citation_signal":8,"citers_with_endowment":8,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":210248,"name":"P. Donini","orcid":null,"position":1,"is_corresponding":false},{"id":210246,"name":"S. Donini","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.639057329615259,"endowment":2.639057329615259,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"5308648","pmcid":null,"openalex_id":"https://openalex.org/W2117618406","authors":[],"funders":[],"total_grants":0,"fwci":0.602,"citation_percentile":0.61644726,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://eje.bioscientifica.com/view/journals/eje/62/1_Suppl/acta_62_Suppl_142_017.xml","host_type":"publisher"},{"url":"https://doi.org/10.1530/acta.0.062s257","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/5308648","host_type":"repository"}],"fields_of_study":["Mass Spectrometry Techniques and Applications","Per- and polyfluoroalkyl substances research","Medicine","Animals","Antigens","Chorionic Gonadotropin","Chromatography","Follicle Stimulating Hormone","Humans","Immune Sera","Immunoelectrophoresis","Iodine Isotopes","Luteinizing Hormone","Radioimmunoassay","Rats","Urine"],"mesh_terms":["Animals","Antigens","Chromatography","Follicle Stimulating Hormone","Chorionic Gonadotropin","Humans","Immune Sera","Immunoelectrophoresis","Iodine Isotopes","Luteinizing Hormone","Radioimmunoassay","Urine","Rats"],"keywords":["Radioimmunoassay","Antiserum","Urine","Chemistry","Urinary system","Endocrinology","Internal medicine","Chromatography","Hormone","Antibody","Biology","Medicine","Immunology"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-01T22:12:24.578480Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}