{"doi":"10.1530/acta.0.0600621","title":"STEROID FORMATION IN PORCINE OVARIAN TISSUE IN VITRO","abstract":"<jats:title>ABSTRACT</jats:title>\n        <jats:p>Ovarian tissue slices from the sow have been incubated simultaneously with pregnenolone and progesterone as substrates, one being labelled with <jats:sup>3</jats:sup>H, the other with <jats:sup>14</jats:sup>C. Progesterone, 17α-hydroxyprogesterone and androstenedione were isolated in a radiochemically pure form; the isotope ratios and the specific activities were determined after chemical quantitation by gas chromatography.</jats:p>\n        <jats:p>Both substrates were used in the biosynthesis of the isolated compounds. The isotope ratios in progesterone, 17α-hydroxy-progesterone and androstenedione were identical or very similar, indicating that androstenedione was formed solely via the progesterone pathway. The results of a time study were in agreement with this conclusion. From the specific activities of the isolated steroids it appeared that the exogenous radioactivity and endogenous substrates were metabolized in a very similar manner.</jats:p>\n        <jats:p>Pregnenolone sulphate was metabolized to the same isolated steroids. The first step in the conversion of this substrate appeared to be removal of the sulphate group.</jats:p>","journal":"Acta Endocrinologica","year":1969,"id":36601,"datarank":0.8170188620349831,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.5051026307830077,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.5051026307830077,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":7,"citers_with_citation_signal":7,"citers_with_endowment":7,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":185023,"name":"Asbjørn Aakvaag","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"5819066","pmcid":null,"openalex_id":"https://openalex.org/W2034235694","authors":[],"funders":[],"total_grants":0,"fwci":0.2504,"citation_percentile":0.53754804,"influential_citations":0,"citation_trend":[{"year":2018,"count":2}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://eje.bioscientifica.com/view/journals/eje/60/4/acta_60_4_008.xml","host_type":"publisher"},{"url":"https://doi.org/10.1530/acta.0.0600621","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/5819066","host_type":"repository"}],"fields_of_study":["Steroid Chemistry and Biochemistry","Estrogen and related hormone effects","Pharmacological Effects and Assays","Medicine","Biology","17-Ketosteroids","Androstanes","Animals","Carbon Isotopes","Cholesterol","Chromatography, Gas","Crystallization","Female","In Vitro Techniques","Ovarian Follicle","Ovary","Pregnenolone","Progesterone","Swine","Tritium"],"mesh_terms":["Androstanes","Animals","Carbon Isotopes","Cholesterol","Chromatography, Gas","Crystallization","Female","Ovarian Follicle","Ovary","Pregnenolone","Progesterone","Swine","Tritium","17-Ketosteroids","In Vitro Techniques"],"keywords":["Androstenedione","Pregnenolone","Chemistry","Steroid","Internal medicine","Endocrinology","In vitro","Estrone","Endogeny","Ovarian tissue","Substrate (aquarium)","Ovary","Hydroxyprogesterone","Isotope","Biosynthesis","Metabolism","Chromatography","Biochemistry","Hormone","Biology","Enzyme","Androgen","Medicine"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-10T16:48:48.445916Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}