{"doi":"10.1530/acta.0.0540527","title":"THE PURIFICATION OF 125I-GLUCAGON OF HIGH SPECIFIC ACTIVITY FOR RADIOIMMUNOCHEMICAL ESTIMATION OF GLUCAGON AND A QUALITATIVE COMPARISON OF GLUCAGON FROM DIFFERENT SOURCES","abstract":"<jats:title>ABSTRACT</jats:title>\n        <jats:p>Radioactively labelled glucagon gave a low percentage binding with antibodies.</jats:p>\n        <jats:p>This was not caused by a low antibody concentration in the antisera, but due to lack of reactivity of a part of the labelled glucagon to the antibodies. The radioactive glucagon was purified therefore by antibody binding and isolation of the antigen-antibody complex by gelfiltration. <jats:sup>125</jats:sup>I-glucagon suitable for radioimmunochemical assay was obtained after dissociation of the complex.</jats:p>\n        <jats:p>Parallelism of response was studied in beef-pork glucagon, pork glucagon, extracts of dog and human pancreas and extracts of human plasma.</jats:p>\n        <jats:p>From the results it was concluded that the antiglucagon serum used did not discriminate between beef-pork, pork, human and dog glucagon.</jats:p>\n        <jats:p>Moreover glucagon was identified radioimmunochemically in extracts of some parts of the human gastrointestinal tract.</jats:p>","journal":"Acta Endocrinologica","year":1967,"id":619505,"datarank":0.37273599746820013,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.0,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1598727,"name":"W. H. L. Hackeng","orcid":null,"position":1,"is_corresponding":false},{"id":1598728,"name":"C. Steendijk","orcid":null,"position":2,"is_corresponding":false},{"id":1598726,"name":"W. Schopman","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"THE PURIFICATION OF 125I-GLUCAGON OF HIGH SPECIFIC ACTIVITY FOR RADIOIMMUNOCHEMICAL ESTIMATION OF GLUCAGON AND A QUALITATIVE COMPARISON OF GLUCAGON FROM DIFFERENT SOURCES","abstract":"<jats:title>ABSTRACT</jats:title>\n        <jats:p>Radioactively labelled glucagon gave a low percentage binding with antibodies.</jats:p>\n        <jats:p>This was not caused by a low antibody concentration in the antisera, but due to lack of reactivity of a part of the labelled glucagon to the antibodies. The radioactive glucagon was purified therefore by antibody binding and isolation of the antigen-antibody complex by gelfiltration. <jats:sup>125</jats:sup>I-glucagon suitable for radioimmunochemical assay was obtained after dissociation of the complex.</jats:p>\n        <jats:p>Parallelism of response was studied in beef-pork glucagon, pork glucagon, extracts of dog and human pancreas and extracts of human plasma.</jats:p>\n        <jats:p>From the results it was concluded that the antiglucagon serum used did not discriminate between beef-pork, pork, human and dog glucagon.</jats:p>\n        <jats:p>Moreover glucagon was identified radioimmunochemically in extracts of some parts of the human gastrointestinal tract.</jats:p>","is_dataset_classified":null,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"6071368","pmcid":null,"openalex_id":"https://openalex.org/W2049734280","authors":[],"funders":[],"total_grants":0,"fwci":3.3272,"citation_percentile":0.91745283,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://eje.bioscientifica.com/view/journals/eje/54/3/acta_54_3_013.xml","host_type":"publisher"},{"url":"https://doi.org/10.1530/acta.0.0540527","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/6071368","host_type":"repository"}],"fields_of_study":["Gastroesophageal reflux and treatments","Radiopharmaceutical Chemistry and Applications","Digestive system and related health","Animals","Antigen-Antibody Reactions","Cattle","Colon","Dogs","Duodenum","Glucagon","Ileum","Immunoassay","In Vitro Techniques","Iodine Isotopes","Jejunum","Pancreatic Extracts","Pylorus","Stomach","Swine","Tissue Extracts"],"mesh_terms":["Animals","Antigen-Antibody Reactions","Cattle","Colon","Dogs","Duodenum","Glucagon","Ileum","Immunoassay","Iodine Isotopes","Jejunum","Pancreatic Extracts","Pylorus","Stomach","Swine","Tissue Extracts","In Vitro Techniques"],"keywords":["Glucagon","Antiserum","Antibody","Internal medicine","Endocrinology","Chemistry","Pancreas","Biology","Hormone","Medicine","Immunology"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Reduced inequalities"},{"sdg_number":0,"sdg_label":"Peace, Justice and strong institutions"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T07:25:53.390723Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}