{"doi":"10.1529/biophysj.103.022087","title":"Photobleaching-Corrected FRET Efficiency Imaging of Live Cells","abstract":null,"journal":"Biophysical Journal","year":2004,"id":46509,"datarank":15.438033411509261,"base_score":6.0063531596017325,"endowment":6.0063531596017325,"self_citation_contribution":0.90095297394026,"citation_network_contribution":14.537080437569001,"self_endowment_contribution":0.90095297394026,"citer_contribution":14.537080437569001,"corpus_percentile":null,"corpus_rank":null,"citation_count":405,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":215594,"name":"Nicholas R.J. 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Furthermore, photobleaching poses a problem for FRET imaging in timelapse experiments and three-dimensional reconstructions. We present a 3-cube FRET imaging method, E-FRET, which overcomes both of these obstacles. E-FRET bridges the gap between the donor recovery after acceptor photobleaching technique (which allows absolute measurements of FRET efficiency, E, but is not suitable for living cells), and the sensitized-emission FRET indices (which reflect FRET in living cells but lack the quantitation and clarity of E). With E-FRET, we visualize FRET in terms of true FRET efficiency images (E), which correlate linearly with the degree of donor interaction. We have defined procedures to incorporate photobleaching correction into E-FRET imaging. We demonstrate the benefits of E-FRET with photobleaching correction for timelapse and three-dimensional imaging of protein-protein interactions in the immunological synapse in living T-cells.","is_dataset_classified":null,"base_score":6.0063531596017325,"endowment":6.0063531596017325,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"15189889","pmcid":"PMC1304294","openalex_id":"https://openalex.org/W2127669953","authors":[],"funders":[{"funder_name":"NIMH NIH HHS","grant_id":"P30 MH062261","title":null},{"funder_name":"NIDA NIH HHS","grant_id":"DA14063","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM065230","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM65230","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"R21 DK061329","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"DK61329","title":null},{"funder_name":"NIMH NIH HHS","grant_id":"MH62261","title":null},{"funder_name":"National Institutes of Health","grant_id":"5P30MH062261-15","title":"Developmental Core (Page 204)"},{"funder_name":"National Institutes of Health","grant_id":"5R01GM065230-10","title":"Intermolecular Interactions in the Immunological Synapse"},{"funder_name":"National Institutes of Health","grant_id":"5R03DA014036-02","title":"Cannabinoid Receptor Dynamics in T Cell Activation"},{"funder_name":"National Institutes of Health","grant_id":"5P30MH062261-17","title":"Chronic HIV Infection and Aging in NeuroAIDS (CHAIN) Center"}],"total_grants":11,"fwci":18.7817,"citation_percentile":0.99366286,"influential_citations":34,"citation_trend":[{"year":2012,"count":20},{"year":2013,"count":15},{"year":2014,"count":14},{"year":2015,"count":19},{"year":2016,"count":16},{"year":2017,"count":15},{"year":2018,"count":19},{"year":2019,"count":32},{"year":2020,"count":25},{"year":2021,"count":17},{"year":2022,"count":11},{"year":2023,"count":22},{"year":2024,"count":19},{"year":2025,"count":22},{"year":2026,"count":10}],"oa_status":"bronze","license":"Elsevier Non-Commercial","oa_locations":[{"url":"http://www.cell.com/article/S0006349504744331/pdf","host_type":"journal"},{"url":"http://www.cell.com/article/S0006349504744331/pdf","host_type":"BRONZE"},{"url":"http://www.cell.com/article/S0006349504744331/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0006349504744331?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0006349504744331?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1529/biophysj.103.022087","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/15189889","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1304294","host_type":"repository"},{"url":"http://dx.doi.org/10.1529/biophysj.103.022087","host_type":""},{"url":"https://dx.doi.org/10.1529/biophysj.103.022087","host_type":""}],"fields_of_study":["Advanced Fluorescence Microscopy Techniques","Advanced Electron Microscopy Techniques and Applications","Retinal Development and Disorders","Chemistry","Medicine","Biology","Engineering","0301 basic medicine","0303 health sciences","03 medical and health sciences","Animals","Cells, Cultured","Cloning, Molecular","Fluorescence Resonance Energy Transfer","Green Fluorescent Proteins","Mice","Models, Theoretical","Photobleaching"],"mesh_terms":["Animals","Cells, Cultured","Cloning, Molecular","Models, Theoretical","Fluorescence Resonance Energy Transfer","Photobleaching","Green Fluorescent Proteins","Mice"],"keywords":["Förster resonance energy transfer","Photobleaching","Live cell imaging","Confocal microscopy","Confocal","Fluorescence-lifetime imaging microscopy","Fluorescence recovery after photobleaching","Biophysics","Microscopy","Chemistry","Fluorescence","Optics","Physics","Biology","Cell","Mice","Green Fluorescent Proteins","Fluorescence Resonance Energy Transfer","Animals","Cloning, Molecular","Models, Theoretical","Cells, Cultured"],"sdg_mappings":[{"sdg_number":7,"sdg_label":"7. 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