{"doi":"10.15252/embj.2023113475","title":"Cohesin‐mediated DNA loop extrusion resolves sister chromatids in G2 phase","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>Genetic information is stored in linear DNA molecules, which are highly folded inside cells. DNA replication along the folded template path yields two sister chromatids that initially occupy the same nuclear region in an intertwined arrangement. Dividing cells must disentangle and condense the sister chromatids into separate bodies such that a microtubule‐based spindle can move them to opposite poles. While the spindle‐mediated transport of sister chromatids has been studied in detail, the chromosome‐intrinsic mechanics presegregating sister chromatids have remained elusive. Here, we show that human sister chromatids resolve extensively already during interphase, in a process dependent on the loop‐extruding activity of cohesin, but not that of condensins. Increasing cohesin's looping capability increases sister DNA resolution in interphase nuclei to an extent normally seen only during mitosis, despite the presence of abundant arm cohesion. That cohesin can resolve sister chromatids so extensively in the absence of mitosis‐specific activities indicates that DNA loop extrusion is a generic mechanism for segregating replicated genomes, shared across different Structural Maintenance of Chromosomes (SMC) protein complexes in all kingdoms of life.</jats:p>","journal":"The EMBO Journal","year":2023,"id":624396,"datarank":0.4943755299006494,"base_score":3.295836866004329,"endowment":3.295836866004329,"self_citation_contribution":0.4943755299006494,"citation_network_contribution":0.0,"self_endowment_contribution":0.4943755299006494,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":26,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":848142,"name":"Christoph C. H. Langer","orcid":"0000-0001-8751-1564","position":1,"is_corresponding":false},{"id":1614125,"name":"Zsuzsanna Takács","orcid":"0000-0003-4741-6487","position":2,"is_corresponding":false},{"id":130290,"name":"Wen Tang","orcid":null,"position":3,"is_corresponding":false},{"id":810616,"name":"Claudia Blaukopf","orcid":null,"position":4,"is_corresponding":false},{"id":241656,"name":"Jan‐Michael Peters","orcid":"0000-0003-2820-3195","position":5,"is_corresponding":false},{"id":557202,"name":"Daniel W. Gerlich","orcid":"0000-0003-1637-3365","position":6,"is_corresponding":false},{"id":16787,"name":"Paul Batty","orcid":"0000-0002-9807-5099","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Cohesin‐mediated DNA loop extrusion resolves sister chromatids in G2 phase","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>Genetic information is stored in linear DNA molecules, which are highly folded inside cells. DNA replication along the folded template path yields two sister chromatids that initially occupy the same nuclear region in an intertwined arrangement. Dividing cells must disentangle and condense the sister chromatids into separate bodies such that a microtubule‐based spindle can move them to opposite poles. While the spindle‐mediated transport of sister chromatids has been studied in detail, the chromosome‐intrinsic mechanics presegregating sister chromatids have remained elusive. Here, we show that human sister chromatids resolve extensively already during interphase, in a process dependent on the loop‐extruding activity of cohesin, but not that of condensins. Increasing cohesin's looping capability increases sister DNA resolution in interphase nuclei to an extent normally seen only during mitosis, despite the presence of abundant arm cohesion. That cohesin can resolve sister chromatids so extensively in the absence of mitosis‐specific activities indicates that DNA loop extrusion is a generic mechanism for segregating replicated genomes, shared across different Structural Maintenance of Chromosomes (SMC) protein complexes in all kingdoms of life.</jats:p>","is_dataset_classified":null,"base_score":3.295836866004329,"endowment":3.295836866004329,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"37357575","pmcid":"PMC10425840","openalex_id":"https://openalex.org/W4382011857","authors":[],"funders":[{"funder_name":"Austrian Research Promotion Agency","grant_id":"FFG‐852936","title":null},{"funder_name":"Austrian Science Fund","grant_id":"DK W1238","title":null},{"funder_name":"EC | European Research Council","grant_id":"101019039","title":"Topological interactions as functional regulators of the eukaryotic genome: moving beyond intramolecular looping"},{"funder_name":"EC | European Research Council","grant_id":"693949","title":"Molecular mechanisms of cohesin-mediated sister chromatid cohesion and chromatin organization"},{"funder_name":"Human Frontier Science Program","grant_id":"RGP0057/2018","title":null},{"funder_name":"Vienna Science and Technology Fund","grant_id":"LS17‐003","title":null},{"funder_name":"Vienna Science and Technology Fund","grant_id":"LS19‐001","title":null},{"funder_name":"Vienna Science and Technology Fund","grant_id":"LS19‐029","title":null},{"funder_name":"Austrian Science Fund (FWF)","grant_id":"W 1238","title":"Vollantrag zu Chromosome Dynamics"}],"total_grants":9,"fwci":2.4113,"citation_percentile":0.89936529,"influential_citations":0,"citation_trend":[{"year":2023,"count":1},{"year":2024,"count":10},{"year":2025,"count":11},{"year":2026,"count":4}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.15252/embj.2023113475","host_type":"journal"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.15252/embj.2023113475","host_type":"publisher"},{"url":"https://link.springer.com/content/pdf/10.15252/embj.2023113475.pdf","host_type":"publisher"},{"url":"https://link.springer.com/article/10.15252/embj.2023113475/fulltext.html","host_type":"publisher"},{"url":"https://www.embopress.org/doi/pdf/10.15252/embj.2023113475","host_type":"publisher"},{"url":"https://doi.org/10.15252/embj.2023113475","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/37357575","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10425840","host_type":"repository"},{"url":"https://doi.org/10.17867/10000191","host_type":""},{"url":"https://doi.org/10.17867/10000191a","host_type":""},{"url":"https://doi.org/10.17867/10000191b","host_type":""},{"url":"https://doi.org/10.17867/10000191c","host_type":""},{"url":"https://doi.org/10.17867/10000191d","host_type":""},{"url":"https://doi.org/10.17867/10000191e","host_type":""},{"url":"https://doi.org/10.17867/10000191f","host_type":""},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10425840/pdf/EMBJ-42-e113475.pdf","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC10425840","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC10425840?pdf=render","host_type":"Europe_PMC"},{"url":"https://doi.org/10.1101/2023.01.12.523718","host_type":""},{"url":"http://dx.doi.org/10.15252/embj.2023113475","host_type":""},{"url":"http://dx.doi.org/10.1101/2023.01.12.523718","host_type":""}],"fields_of_study":["Genomics and Chromatin Dynamics","DNA and Nucleic Acid Chemistry","RNA and protein synthesis mechanisms","0301 basic medicine","03 medical and health sciences"],"mesh_terms":["Cohesins","Chromatids","Chromosomal Proteins, Non-Histone","DNA","Humans","Mitosis","G2 Phase","Cell Cycle Proteins"],"keywords":["Cohesin","Biology","Sister chromatids","Establishment of sister chromatid cohesion","Genetics","DNA","Cell biology","Chromosome","Gene","Chromatin","Mitosis","chromosomes","Condensin","Sister Chromatid Resolution","G2 Phase","Chromosomal Proteins, Non-Histone","Humans","Cell Cycle Proteins","Articles","Chromatids","Cohesins"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Quality Education"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"},{"name":"doi"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-04T03:39:33.119312Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}