{"doi":"10.15252/embj.2022112542","title":"Coronaviral ORF6 protein mediates inter‐organelle contacts and modulates host cell lipid flux for virus production","abstract":null,"journal":"The EMBO Journal","year":2023,"id":675421,"datarank":1.5686647597712147,"base_score":4.189654742026425,"endowment":4.189654742026425,"self_citation_contribution":0.6284482113039639,"citation_network_contribution":0.9402165484672508,"self_endowment_contribution":0.6284482113039639,"citer_contribution":0.9402165484672508,"corpus_percentile":null,"corpus_rank":null,"citation_count":65,"citer_count":61,"citers_with_citation_signal":44,"citers_with_endowment":44,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":763036,"name":"Bing Hu","orcid":"0000-0001-6408-5240","position":1,"is_corresponding":false},{"id":465927,"name":"Jiajia Li","orcid":"0009-0005-7848-0897","position":2,"is_corresponding":false},{"id":903141,"name":"Ruifeng Chen","orcid":"0000-0002-0466-1266","position":3,"is_corresponding":false},{"id":747361,"name":"Zhen Yuan","orcid":"0000-0003-3061-6263","position":4,"is_corresponding":false},{"id":1764739,"name":"Hurong Xiao","orcid":null,"position":5,"is_corresponding":false},{"id":1764740,"name":"Haishuang Chang","orcid":null,"position":6,"is_corresponding":false},{"id":460714,"name":"Yaming Jiu","orcid":"0000-0002-8601-8820","position":7,"is_corresponding":false},{"id":1496482,"name":"Kun Cai","orcid":"0000-0002-0070-5954","position":8,"is_corresponding":false},{"id":1764741,"name":"Binbin Ding","orcid":"0000-0002-6424-005X","position":9,"is_corresponding":false},{"id":1764736,"name":"Mengzhen Yue","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Coronaviral ORF6 protein mediates inter‐organelle contacts and modulates host cell lipid flux for virus production","abstract":"Lipid droplets (LDs) form inter-organelle contacts with the endoplasmic reticulum (ER) that promote their biogenesis, while LD contacts with mitochondria enhance β-oxidation of contained fatty acids. Viruses have been shown to take advantage of lipid droplets to promote viral production, but it remains unclear whether they also modulate the interactions between LDs and other organelles. Here, we showed that coronavirus ORF6 protein targets LDs and is localized to the mitochondria-LD and ER-LD contact sites, where it regulates LD biogenesis and lipolysis. At the molecular level, we find that ORF6 inserts into the LD lipid monolayer via its two amphipathic helices. ORF6 further interacts with ER membrane proteins BAP31 and USE1 to mediate ER-LDs contact formation. Additionally, ORF6 interacts with the SAM complex in the mitochondrial outer membrane to link mitochondria to LDs. In doing so, ORF6 promotes cellular lipolysis and LD biogenesis to reprogram host cell lipid flux and facilitate viral production.","is_dataset_classified":null,"base_score":4.189654742026425,"endowment":4.189654742026425,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"37218505","pmcid":"PMC10308351","openalex_id":"https://openalex.org/W4377565369","authors":[],"funders":[{"funder_name":"MOST | National Natural Science Foundation of China","grant_id":"92054107","title":null},{"funder_name":"MOST | National Natural Science Foundation of China","grant_id":"U22A20337","title":null},{"funder_name":"MOST | National Natural Science Foundation of China","grant_id":"92054104","title":null},{"funder_name":"MOST | National Natural Science Foundation of China","grant_id":"32222022","title":null},{"funder_name":"Open Research Fund Program of the State Key Laboratory of Virology of China","grant_id":"2022KF006","title":null}],"total_grants":5,"fwci":8.3777,"citation_percentile":0.98605472,"influential_citations":0,"citation_trend":[{"year":2023,"count":6},{"year":2024,"count":16},{"year":2025,"count":24},{"year":2026,"count":19}],"oa_status":"hybrid","license":"other-oa","oa_locations":[{"url":"https://www.embopress.org/action/downloadSupplement?doi=10.15252/embj.2022112542&file=embj2022112542.reviewer_comments.pdf","host_type":"journal"},{"url":"https://www.embopress.org/action/downloadSupplement?doi=10.15252/embj.2022112542&file=embj2022112542.reviewer_comments.pdf","host_type":"publisher"},{"url":"https://link.springer.com/content/pdf/10.15252/embj.2022112542.pdf","host_type":"publisher"},{"url":"https://link.springer.com/article/10.15252/embj.2022112542/fulltext.html","host_type":"publisher"},{"url":"https://www.embopress.org/doi/pdf/10.15252/embj.2022112542","host_type":"publisher"},{"url":"https://doi.org/10.15252/embj.2022112542","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/37218505","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10308351","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10308351/pdf/EMBJ-42-e112542.pdf","host_type":"repository"}],"fields_of_study":["Lipid metabolism and biosynthesis","Endoplasmic Reticulum Stress and Disease","Pancreatic function and diabetes"],"mesh_terms":["Endoplasmic Reticulum","Fatty Acids","Lipolysis","Coronavirus","Lipid Droplets"],"keywords":["Lipid droplet","Biology","Endoplasmic reticulum","Cell biology","Organelle","Membrane contact site","Biogenesis","Mitochondrion","Organelle biogenesis","Membrane protein","Biochemistry","Membrane","Integral membrane protein","Gene","Mitochondria","lipid droplets","Orf6","Organelle Interaction"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"},{"name":"doi"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T00:29:41.554880Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}