{"doi":"10.15252/embj.2021108747","title":"Keratin filaments mediate the expansion of extra‐embryonic membranes in the post‐gastrulation mouse embryo","abstract":null,"journal":"The EMBO Journal","year":2022,"id":615502,"datarank":0.4636563680037475,"base_score":3.091042453358316,"endowment":3.091042453358316,"self_citation_contribution":0.4636563680037475,"citation_network_contribution":0.0,"self_endowment_contribution":0.4636563680037475,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":21,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":860804,"name":"Sema Elif Eski","orcid":"0000-0002-4757-8614","position":1,"is_corresponding":false},{"id":490771,"name":"Evangéline Despin‐Guitard","orcid":"0000-0002-5928-6387","position":2,"is_corresponding":false},{"id":303138,"name":"Marjorie Vermeersch","orcid":null,"position":3,"is_corresponding":false},{"id":1586503,"name":"Marie Versaevel","orcid":"0000-0003-0710-4243","position":4,"is_corresponding":false},{"id":604637,"name":"Bechara Saykali","orcid":"0000-0002-9097-687X","position":5,"is_corresponding":false},{"id":992389,"name":"Daniel Monteyne","orcid":"0000-0002-2529-2455","position":6,"is_corresponding":false},{"id":839345,"name":"Sylvain Gabriele","orcid":"0000-0001-8696-8052","position":7,"is_corresponding":false},{"id":692874,"name":"Thomas M. 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Two-photon live imaging of embryos bearing a keratin reporter allowed recording filament nucleation and elongation in the extra-embryonic region. Upon separation of amniotic and exocoelomic cavities, keratin 8 formed apical cables co-aligned across multiple cells in the amnion, allantois, and blood islands. An influence of substrate rigidity and composition on cell behavior and keratin content was observed in mesoderm explants. Embryos lacking all keratin filaments displayed a deflated extra-embryonic cavity, a narrow thick amnion, and a short allantois. Single-cell RNA sequencing of sorted mesoderm cells and micro-dissected amnion, chorion, and allantois, provided an atlas of transcriptomes with germ layer and regional information. It defined the cytoskeleton and adhesion expression profile of mesoderm-derived keratin 8-enriched cells lining the exocoelomic cavity. Those findings indicate a novel role for keratin filaments in the expansion of extra-embryonic structures and suggest mechanisms of mesoderm adaptation to the environment.","is_dataset_classified":null,"base_score":3.091042453358316,"endowment":3.091042453358316,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"35266581","pmcid":"PMC8982622","openalex_id":"https://openalex.org/W4220783520","authors":[],"funders":[{"funder_name":"WELBIO","grant_id":"SGR2015","title":null},{"funder_name":"FEDER Prostem","grant_id":"1510614","title":null},{"funder_name":"Fonds De La Recherche Scientifique ‐ FNRS","grant_id":"PDR T.0084.16","title":null},{"funder_name":"Fonds De La Recherche Scientifique ‐ FNRS","grant_id":"MISU 34772792","title":null},{"funder_name":"Fonds De La Recherche Scientifique ‐ FNRS","grant_id":"40006730","title":null},{"funder_name":"Fonds De La Recherche Scientifique ‐ 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