{"doi":"10.1523/jneurosci.0929-20.2020","title":"Myosin X Interaction with KIF13B, a Crucial Pathway for Netrin-1-Induced Axonal Development","abstract":"Myosin X (Myo X) transports cargos to the tips of filopodia for cell adhesion, migration, and neuronal axon guidance. Deleted in Colorectal Cancer (DCC) is one of the Myo X cargos that is essential for Netrin-1-regulated axon pathfinding. The function of Myo X in axon development in vivo and the underlying mechanisms remain elusive. Here, we provide evidence for the role of Myo X in Netrin-1-DCC-regulated axon development in developing mouse neocortex. The knockout (KO) or knockdown (KD) of Myo X in cortical neurons of embryonic mouse brain impairs axon initiation and contralateral branching/targeting. Similar axon deficits are detected in Netrin-1-KO or DCC-KD cortical neurons. Further proteomic analysis of Myo X binding proteins identifies KIF13B (a kinesin family motor protein). The Myo X interaction with KIF13B is induced by Netrin-1. Netrin-1 promotes anterograde transportation of Myo X into axons in a KIF13B-dependent manner. KIF13B-KD cortical neurons exhibit similar axon deficits. Together, these results reveal Myo X-KIF13B as a critical pathway for Netrin-1-promoted axon initiation and branching/targeting. SIGNIFICANCE STATEMENT Netrin-1 increases Myosin X (Myo X) interaction with KIF13B, and thus promotes axonal delivery of Myo X and axon initiation and contralateral branching in developing cerebral neurons, revealing unrecognized functions and mechanisms underlying Netrin-1 regulation of axon development.","journal":"Journal of Neuroscience","year":2020,"id":69377,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":21,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9536,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":367391,"name":"Yun Peng","orcid":"0000-0001-8476-5593","position":1,"is_corresponding":false},{"id":353306,"name":"Yang Zhao","orcid":"0000-0003-4524-6154","position":2,"is_corresponding":false},{"id":367392,"name":"Yongsheng Lan","orcid":"0000-0003-3419-3544","position":3,"is_corresponding":false},{"id":367393,"name":"Bo Wang","orcid":"0000-0002-1310-869X","position":4,"is_corresponding":false},{"id":367394,"name":"Lu Zhao","orcid":"0000-0003-3918-5711","position":5,"is_corresponding":false},{"id":330608,"name":"Dong Sun","orcid":"0000-0003-1852-6572","position":6,"is_corresponding":false},{"id":289855,"name":"Jin‐Xiu Pan","orcid":"0000-0003-4013-1390","position":7,"is_corresponding":false},{"id":120960,"name":"Zhaoqi Dong","orcid":null,"position":8,"is_corresponding":false},{"id":289863,"name":"Lin Mei","orcid":"0000-0001-5772-1229","position":9,"is_corresponding":false},{"id":367395,"name":"Yu‐Qiang Ding","orcid":"0000-0003-1202-4635","position":10,"is_corresponding":false},{"id":355090,"name":"Xiaojuan Zhu","orcid":"0000-0001-8386-8064","position":11,"is_corresponding":false},{"id":330611,"name":"Wen‐Cheng Xiong","orcid":"0000-0001-9071-7598","position":12,"is_corresponding":false},{"id":368209,"name":"Hua‐Li Yu","orcid":null,"position":0,"is_corresponding":true}],"reference_count":61,"raw_metadata":null,"created_at":"2026-07-18T21:42:22.705573Z","pmid":"33097641","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}