{"doi":"10.1515/jpm-2022-0504","title":"Rapid diagnosis of intra-amniotic infection using nanopore-based sequencing","abstract":"OBJECTIVES: Early diagnosis and treatment of intra-amniotic infection is crucial. Rapid pathogen identification allows for a definite diagnosis and enables proper management. We determined whether the 16S amplicon sequencing performed by a nanopore sequencing technique make possible rapid bacterial identification at the species level in intra-amniotic infection. METHODS: Five cases of confirmed intra-amniotic infection, determined by either cultivation or 16S rDNA polymerase chain reaction (PCR) Sanger sequencing, and 10 cases of women who underwent mid-trimester genetic amniocentesis were included. DNA was extracted from amniotic fluid and PCR was performed on the full-length 16S rDNA. Nanopore sequencing was performed. The results derived from nanopore sequencing were compared with those derived from cultivation and Sanger sequencing methods. RESULTS: Bacteria were successfully detected from amniotic fluid using nanopore sequencing in all cases of intra-amniotic infection. Nanopore sequencing identified additional bacterial species and polymicrobial infections. All patients who underwent a mid-trimester amniocentesis had negative cultures, negative 16S PCR Sanger sequencing and nanopore sequencing. Identification of the microorganisms using nanopore sequencing technique at the bacterial species level was achieved within 5-9 h from DNA extraction. CONCLUSIONS: This is the first study demonstrating that the nanopore sequencing technique is capable of rapid diagnosis of intra-amniotic infection using fresh amniotic fluid samples.","journal":"Journal of Perinatal Medicine","year":2022,"id":260485,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.96,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":236857,"name":"Roberto Romero","orcid":"0000-0002-4448-5121","position":1,"is_corresponding":false},{"id":914204,"name":"Pisut Pongchaikul","orcid":"0000-0001-8757-3554","position":2,"is_corresponding":false},{"id":914205,"name":"Pornpun Vivithanaporn","orcid":"0000-0002-2639-9545","position":3,"is_corresponding":false},{"id":914719,"name":"Waranyu Lertrut","orcid":null,"position":4,"is_corresponding":false},{"id":840646,"name":"Adithep Jaovisidha","orcid":null,"position":5,"is_corresponding":false},{"id":914720,"name":"Paninee Mongkolsuk","orcid":null,"position":6,"is_corresponding":false},{"id":914206,"name":"Perapon Nitayanon","orcid":"0000-0001-7964-7285","position":7,"is_corresponding":false},{"id":914721,"name":"Khontawan Pongsuktavorn","orcid":null,"position":8,"is_corresponding":false},{"id":914207,"name":"Threebhorn Kamlungkuea","orcid":"0000-0001-7204-1387","position":9,"is_corresponding":false},{"id":240935,"name":"Eunjung Jung","orcid":"0000-0003-0897-0039","position":10,"is_corresponding":false},{"id":841294,"name":"Manaphat Suksai","orcid":"0000-0003-4441-4988","position":11,"is_corresponding":false},{"id":914722,"name":"Arunee Singhsnaeh","orcid":null,"position":12,"is_corresponding":false},{"id":233667,"name":"Piroon Jenjaroenpun","orcid":"0000-0002-1555-401X","position":13,"is_corresponding":false},{"id":494147,"name":"Iyarit Thaipisuttikul","orcid":"0009-0007-0242-0171","position":14,"is_corresponding":false},{"id":233668,"name":"Thidathip Wongsurawat","orcid":"0000-0002-3659-2074","position":15,"is_corresponding":false},{"id":839248,"name":"Piya Chaemsaithong","orcid":"0000-0003-1750-9834","position":0,"is_corresponding":true}],"reference_count":21,"raw_metadata":null,"created_at":"2026-07-19T00:26:07.666421Z","pmid":"36503654","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}