{"doi":"10.1515/dx-2022-0125","title":"Quantitation of neurofilament light chain protein in serum and cerebrospinal fluid from patients with multiple sclerosis using the MSD R-PLEX NfL assay","abstract":"Abstract Objectives Neurofilament light (NfL) chain is a marker of neuroaxonal damage in various neurological diseases. Here we quantitated NfL levels in the cerebrospinal fluid (CSF) and serum from patients with multiple sclerosis (MS) and controls, using the R-PLEX NfL assay, which employs advanced Meso Scale Discovery ® (MSD) electrochemiluminescence (ECL)-based detection technology. Methods NfL was quantitated in samples from 116 individuals from two sites (Ottawa Hospital Research Institute and Mayo Clinic), consisting of patients with MS (n=71) and age- and sex-matched inflammatory neurological controls (n=13) and non-inflammatory controls (n=32). Correlation of NfL levels between CSF and serum was assessed in paired samples in a subset of MS patients and controls (n=61). Additionally, we assessed the correlation between NfL levels obtained with MSD’s R-PLEX ® and Quanterix’s single molecule array (Simoa ® ) assays in CSF and serum (n=32). Results Using the R-PLEX, NfL was quantitated in 99% of the samples tested, and showed a broad range in the CSF (82–500,000 ng/L) and serum (8.84–2,014 ng/L). Nf-L levels in both biofluids correlated strongly (r=0.81, p<0.0001). Lastly, Nf-L measured by MSD’s R-PLEX and Quanterix’s Simoa assays were highly correlated for both biofluids (CSF: r=0.94, p<0.0001; serum: r=0.95, p<0.0001). Conclusions We show that MSD’s R-PLEX NfL assay can reliably quantitate levels of NfL in the CSF and serum from patients with MS and controls, where levels correlate strongly with Simoa.","journal":"Diagnosis","year":2023,"id":345670,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9569,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":975819,"name":"Dorsa Sohaei","orcid":"0000-0003-4824-4277","position":1,"is_corresponding":false},{"id":1085646,"name":"Simon Thebault","orcid":"0000-0003-0395-3395","position":2,"is_corresponding":false},{"id":1086107,"name":"Oscar D. Pons-Belda","orcid":null,"position":3,"is_corresponding":false},{"id":1085647,"name":"Amaia Fernandez-Uriarte","orcid":"0000-0003-2667-7039","position":4,"is_corresponding":false},{"id":1024271,"name":"Christopher Campbell","orcid":"0000-0001-5928-0463","position":5,"is_corresponding":false},{"id":1086108,"name":"David Cheo","orcid":null,"position":6,"is_corresponding":false},{"id":975821,"name":"Martin Stengelin","orcid":"0000-0002-7298-2213","position":7,"is_corresponding":false},{"id":287956,"name":"George B. Sigal","orcid":"0000-0001-7149-6400","position":8,"is_corresponding":false},{"id":335181,"name":"Mark S. Freedman","orcid":"0000-0003-1255-9701","position":9,"is_corresponding":false},{"id":504825,"name":"Isobel A. Scarisbrick","orcid":"0000-0003-2395-589X","position":10,"is_corresponding":false},{"id":975823,"name":"Ioannis Prassas","orcid":"0000-0002-7907-4576","position":11,"is_corresponding":false},{"id":786,"name":"Eleftherios P. Diamandis","orcid":"0000-0002-1589-820X","position":12,"is_corresponding":false},{"id":856416,"name":"Antigona Ulndreaj","orcid":"0000-0003-1650-1187","position":0,"is_corresponding":true}],"reference_count":14,"raw_metadata":null,"created_at":"2026-07-19T01:11:44.152490Z","pmid":"36788117","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}