{"doi":"10.1515/cclm.1996.34.5.431","title":"Simultaneous Automated Determination of Glucose 6-Phosphate Dehydrogenase and 6-Phosphogluconate Dehydrogenase Activities in Whole Blood","abstract":null,"journal":"cclm","year":1996,"id":602212,"datarank":0.4335557636844247,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"self_citation_contribution":0.4335557636844247,"citation_network_contribution":0.0,"self_endowment_contribution":0.4335557636844247,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1544388,"name":"Renata Paleari","orcid":null,"position":1,"is_corresponding":false},{"id":1544389,"name":"Enrico Rosti","orcid":null,"position":2,"is_corresponding":false},{"id":1544390,"name":"Massimo Luzzana","orcid":null,"position":3,"is_corresponding":false},{"id":533392,"name":"Susanna Barella","orcid":"0000-0002-1952-7836","position":4,"is_corresponding":false},{"id":1544391,"name":"Carla Sollaino","orcid":null,"position":5,"is_corresponding":false},{"id":1544392,"name":"Renzo Galanello","orcid":null,"position":6,"is_corresponding":false},{"id":1544387,"name":"Andrea Mosca","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Simultaneous Automated Determination of Glucose 6-Phosphate Dehydrogenase and 6-Phosphogluconate Dehydrogenase Activities in Whole Blood","abstract":"We report a potentiometric fully automated method for determining red cell glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase activities and the glucose 6-phosphate dehydrogenase/6-phosphogluconate dehydrogenase index using 25 microliters of whole blood. No sample pre-treatment (e.g., preparation of the haemolysate) is needed and the measurements are performed at pH 8.0 and 37 degrees C under the conditions recommended by the ICSH committee. The reproducibility was constantly good, with within-run CV of 1.0% (glucose 6-phosphate dehydrogenase) and 5.9% (glucose 6-phosphate dehydrogenase/6-phosphogluconate dehydrogenase) for activities in glucose 6-phosphate dehydrogenase non-deficient adults, and of 2.3% (glucose 6-phosphate dehydrogenase, G6PD) and 2.5% (glucose 6-phosphate dehydrogenase/6-phosphogluconate dehydrogenase) for G6PDMediterranean heterozygotes. Linearity was observed up to an activity of 2800 U/l of glucose 6-phosphate dehydrogenase. Results of glucose 6-phosphate dehydrogenase activity (U/l) in whole blood (y) correlated well with those obtained with the previously described monostarter assay, performed at pH 9.2 (y = 0.60x + 37; n = 80; r = 0.991). Results of 6-phosphogluconate dehydrogenase (U/l) in whole blood (y) correlated well with those obtained by the ICSH recommended method (x) (y = 0.779x - 44; n = 23; r = 0.991). Reference intervals are reported for glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and glucose 6-phosphate dehydrogenase/6-phosphogluconate dehydrogenase index relatively to normal, beta- and alpha-thalassaemic glucose 6-phosphate dehydrogenase non-deficient adults, to glucose 6-phosphate dehydrogenase deficient adult males and to G6PDMediterranean non-thalassaemic heterozygotes. We demonstrate that the diagnostic sensitivity of the glucose 6-phosphate dehydrogenase/6-phosphogluconate dehydrogenase index in detecting the G6PDMediterranean heterozygotes is superior to that of the glucose 6-phosphate dehydrogenase activity alone.","is_dataset_classified":null,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8790979","pmcid":null,"openalex_id":"https://openalex.org/W2135512811","authors":[],"funders":[],"total_grants":0,"fwci":0.3043,"citation_percentile":0.59108734,"influential_citations":0,"citation_trend":[{"year":2016,"count":1},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2022,"count":1},{"year":2023,"count":3},{"year":2024,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://edoc.hu-berlin.de/bitstreams/66491dbd-bf19-4266-86d6-3b65f911629a/download","host_type":"repository"},{"url":"https://edoc.hu-berlin.de/bitstreams/66491dbd-bf19-4266-86d6-3b65f911629a/download","host_type":"repository"},{"url":"https://www.degruyter.com/document/doi/10.1515/cclm.1996.34.5.431/xml","host_type":"publisher"},{"url":"https://www.degruyter.com/document/doi/10.1515/cclm.1996.34.5.431/pdf","host_type":"publisher"},{"url":"http://edoc.hu-berlin.de/18452/11597","host_type":"repository"},{"url":"https://doi.org/10.1515/cclm.1996.34.5.431","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8790979","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.633.2391","host_type":""}],"fields_of_study":["Metabolism and Genetic Disorders","Neonatal Health and Biochemistry","Sexual Differentiation and Disorders"],"mesh_terms":["Adolescent","Adult","Base Sequence","Blood Chemical Analysis","DNA","Evaluation Studies as Topic","Female","Glucosephosphate Dehydrogenase","Glucosephosphate Dehydrogenase Deficiency","Heterozygote","Humans","Male","Phosphogluconate Dehydrogenase","Potentiometry","Sensitivity and Specificity","Genetic Variation","Reproducibility of Results","alpha-Thalassemia","beta-Thalassemia"],"keywords":["Dehydrogenase","Phosphogluconate dehydrogenase","Glucose-6-phosphate dehydrogenase","Biochemistry","Branched-chain alpha-keto acid dehydrogenase complex","Chemistry","Enzyme"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T18:40:57.421315Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}