{"doi":"10.1515/bc.2011.083","title":"Evidence that a septin diffusion barrier is dispensable for cytokinesis in budding yeast","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>Septins are essential for cytokinesis in <jats:italic>Saccharomyces cerevisiae</jats:italic>, but their precise roles remain elusive. Currently, it is thought that before cytokinesis, the hourglass-shaped septin structure at the mother-bud neck acts as a scaffold for assembly of the actomyosin ring (AMR) and other cytokinesis factors. At the onset of cytokinesis, the septin hourglass splits to form a double ring that sandwiches the AMR and may function as diffusion barriers to restrict diffusible cytokinesis factors to the division site. Here, we show that in cells lacking the septin Cdc10 or the septin-associated protein Bud4, the septins form a ring-like structure at the mother-bud neck that fails to re-arrange into a double ring early in cytokinesis. Strikingly, AMR assembly and constriction, the localization of membrane-trafficking and extracellular-matrix-remodeling factors, cytokinesis, and cell-wall-septum formation all occur efficiently in <jats:italic>cdc10Δ</jats:italic> and <jats:italic>bud4Δ</jats:italic> mutants. Thus, diffusion barriers formed by the septin double ring do not appear to be critical for <jats:italic>S. cerevisiae</jats:italic> cytokinesis. However, an AMR mutation and a septin mutation have synergistic effects on cytokinesis and the localization of cytokinesis proteins, suggesting that tethering to the AMR and a septin diffusion barrier may function redundantly to localize proteins to the division site.</jats:p>","journal":"bchm","year":2011,"id":42629,"datarank":2.456270028294222,"base_score":4.477336814478207,"endowment":4.477336814478207,"self_citation_contribution":0.6716005221717312,"citation_network_contribution":1.784669506122491,"self_endowment_contribution":0.6716005221717312,"citer_contribution":1.784669506122491,"corpus_percentile":null,"corpus_rank":null,"citation_count":87,"citer_count":68,"citers_with_citation_signal":57,"citers_with_endowment":57,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":203796,"name":"Ryuichi Nishihama","orcid":null,"position":1,"is_corresponding":false},{"id":202524,"name":"Masayuki Onishi","orcid":"0000-0002-9799-4501","position":2,"is_corresponding":false},{"id":203797,"name":"Younghoon Oh","orcid":null,"position":3,"is_corresponding":false},{"id":203798,"name":"Julia Hanna","orcid":null,"position":4,"is_corresponding":false},{"id":202527,"name":"John R. Pringle","orcid":null,"position":5,"is_corresponding":false},{"id":203799,"name":"Michael Krauß","orcid":null,"position":6,"is_corresponding":false},{"id":201895,"name":"Erfei Bi","orcid":"0000-0002-2998-3608","position":7,"is_corresponding":false},{"id":203795,"name":"Carsten Wloka","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.477336814478207,"endowment":4.477336814478207,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21824009","pmcid":null,"openalex_id":"https://openalex.org/W2046881166","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM087365","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM31006","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM87365","title":null}],"total_grants":3,"fwci":2.2613,"citation_percentile":0.88020261,"influential_citations":5,"citation_trend":[{"year":2012,"count":6},{"year":2013,"count":5},{"year":2014,"count":4},{"year":2015,"count":8},{"year":2016,"count":10},{"year":2017,"count":3},{"year":2018,"count":7},{"year":2019,"count":3},{"year":2020,"count":8},{"year":2021,"count":4},{"year":2022,"count":2},{"year":2023,"count":7},{"year":2024,"count":10},{"year":2025,"count":8}],"oa_status":"closed","license":null,"oa_locations":[{"url":"http://www.degruyter.com/view/j/bchm.2011.392.issue-8-9/bc.2011.083/bc.2011.083.xml","host_type":"publisher"},{"url":"https://www.degruyter.com/document/doi/10.1515/BC.2011.083/xml","host_type":"publisher"},{"url":"https://www.degruyter.com/document/doi/10.1515/BC.2011.083/pdf","host_type":"publisher"},{"url":"https://doi.org/10.1515/bc.2011.083","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/21824009","host_type":"repository"}],"fields_of_study":["Fungal and yeast genetics research","Genetic Neurodegenerative Diseases","Fermentation and Sensory Analysis","Chemistry","Medicine","Biology","Biological Transport","Cell Membrane","Chitin Synthase","Cytokinesis","Saccharomycetales","Septins"],"mesh_terms":["Biological Transport","Cell Membrane","Chitin Synthase","Saccharomycetales","Cytokinesis","Septins"],"keywords":["Septin","Cytokinesis","Cell biology","Cell division","Saccharomyces cerevisiae","Biology","Chemistry","Yeast","Genetics","Cell"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-14T04:18:56.347958Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}