{"doi":"10.14814/phy2.70205","title":"Response of dorsal horn neurons in mice to high‐frequency (kHz) biphasic stimulation is not sensitive to local temperature rise","abstract":"Clinically accepted for treatment of chronic pain 10 kHz-frequency electric spinal cord stimulation (10 kHz-SCS) releases more power in tissue compared to conventional low-frequency (<100 Hz) stimulation due to increased duty cycle. This is equivalent to the release of more heat in a surrounding tissue, which may change the functional state of affected neural elements. In the case of SCS, plausible candidates to be affected by thermal a component of kHz-frequency electric field stimulation (kHz-FS) are dorsal column axons and neurons of the superficial layers of the dorsal horn. In this study, we tested the hypothesis that joule heat produced by kHz-FS modulates neuronal excitability. In slices of the mouse spinal cord, we monitored membrane potential and membrane input resistance in neurons of lamina II during exposure to kHz-FS. Surprisingly, we found no correlation between temperature rise and changes of membrane parameters. Furthermore, the depolarizing effect of kHz-FS was always immediate and remained persistent throughout stimulation, whereas rise of temperature was delayed for 1-2 s and reached its saturation level within the following few seconds. Thus, we concluded that the thermal component has an insignificant role in the mechanism of kHz-FS action.","journal":"Physiological Reports","year":2025,"id":540303,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9554,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":639803,"name":"Stephanie L. Daugherty","orcid":null,"position":1,"is_corresponding":false},{"id":832847,"name":"Changfeng Tai","orcid":"0000-0002-7776-8611","position":2,"is_corresponding":false},{"id":421855,"name":"Naoki Yoshimura","orcid":"0000-0001-8070-1664","position":3,"is_corresponding":false},{"id":441550,"name":"Sergei Karnup","orcid":"0000-0001-7757-5046","position":0,"is_corresponding":true}],"reference_count":52,"raw_metadata":null,"created_at":"2026-07-19T02:52:38.861025Z","pmid":"39916294","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}