{"doi":"10.14814/phy2.70120","title":"T cell‐expressed <i>Ift88</i> is required for proper thymocyte differentiation in mice","abstract":"Abstract Intraflagellar transport protein 88 ( Ift88 ) is required for the formation of cilia in the thymus and non‐ciliary dependent functions including T cell immune synapse formation. To test the role of Ift88 in T cell development, we performed flow cytometry analysis on thymus and spleen tissue isolated from mice lacking Ift88 in thymic epithelial cells (TECs) or T cells. Analyses indicated that TEC Ift88 deletion had no impact on thymic T cell development and minimal impact on splenic T cells. Analysis of T cells in CaggCre ERT2+ Ift88 tm1Bky mTmG mice indicate that approximately half of DN1 thymocytes are Ift88 deficient 3 weeks post‐tamoxifen induction; Ift88 loss did not impact T cell development at the DN2‐DN4 stage or the CD4+/CD8+ double‐positive (DP) thymocyte stage. However, survival of Ift88 deficient T cells was significantly reduced at the single‐positive (SP) thymocyte stage, as was the number of CD4+ and CD8+ T cells in spleen and kidney. Despite preferential survival of Ift88 ‐proficient cells, the total number of T cells the in spleen and kidney was minimally impacted by Ift88 loss. These data suggest Ift88 is required for differentiation of DP thymocytes into SP thymocytes and that Ift88 proficient T cells can compensate for deficient cells to fill the open niche.","journal":"Physiological Reports","year":2024,"id":482283,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9542,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":665104,"name":"Nancy M. Gonzalez","orcid":"0000-0002-0354-9598","position":1,"is_corresponding":false},{"id":1070804,"name":"Morgan E. Smith","orcid":"0009-0002-6582-8645","position":2,"is_corresponding":false},{"id":583549,"name":"Mandy J. Croyle","orcid":"0000-0001-5208-0530","position":3,"is_corresponding":false},{"id":371213,"name":"Bradley K. Yoder","orcid":"0000-0002-0247-1944","position":4,"is_corresponding":false},{"id":15661,"name":"Kurt A. Zimmerman","orcid":"0000-0003-1798-8046","position":5,"is_corresponding":false},{"id":15656,"name":"Sarah J. Miller","orcid":"0000-0003-4888-5306","position":0,"is_corresponding":true}],"reference_count":38,"raw_metadata":null,"created_at":"2026-07-19T02:07:14.349221Z","pmid":"39562155","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}