{"doi":"10.14670/hh-22.1077","title":"Unknown","abstract":null,"journal":null,"year":null,"id":597184,"datarank":0.37273599746820013,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.0,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The absence of one or both nidogens does not alter basement membrane composition in adult murine kidney.","abstract":"Nidogen-1 and nidogen-2 are major components of all basement membranes and are considered to function as link molecules between laminin and collagen type IV networks. Surprisingly, the knockout of one or both nidogens does not cause defects in all tissues or in all basement membranes. In this study, we have elucidated the appearance of the major basement membrane components in adult murine kidney lacking nidogen-1, nidogen-2, or both nidogens. To this end, we localized laminin-111, perlecan, and collagen type IV in knockout mice, heterozygous (+/-) or homozygous (-/-) for the nidogen-1 gene, the nidogen-2 gene, or both nidogen genes with the help of light microscopic immunostaining. We also performed immunogold histochemistry to determine the occurrence of these molecules in the murine kidney at the ultrastructural level. The renal basement membranes of single knockout mice contained a similar distribution of laminin-111, perlecan, and collagen type IV compared to heterozygous mice. In nidogen double-knockout animals, the basement membrane underlying the tubular epithelium was sometimes altered, giving a diffuse and thickened pattern, or was totally absent. The normal or thickened basement membrane of double-knockout mice also showed a similar distribution of laminin-111, perlecan, and collagen type IV. The results indicate that the lack of nidogen-1, nidogen-2, or both nidogens, plays no crucial role in the occurrence and localization of laminin-111, collagen type IV, and perlecan in murine tubular renal basement membranes.","is_dataset_classified":null,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17616934","pmcid":null,"openalex_id":"https://openalex.org/W111035194","authors":[],"funders":[],"total_grants":0,"fwci":0.5819,"citation_percentile":0.64224619,"influential_citations":0,"citation_trend":[{"year":2015,"count":1},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2018,"count":2},{"year":2019,"count":1},{"year":2023,"count":1}],"oa_status":"green","license":"other-oa","oa_locations":[{"url":"http://hdl.handle.net/10201/27639","host_type":"repository"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17616934","host_type":"repository"}],"fields_of_study":["Cell Adhesion Molecules Research","Galectins and Cancer Biology","Bone and Dental Protein Studies"],"mesh_terms":["Animals","Calcium-Binding Proteins","Immunoenzyme Techniques","Kidney","Laminin","Membrane Glycoproteins","Cell Adhesion Molecules","Mice, Knockout","Heparan Sulfate Proteoglycans","Gene Silencing","Collagen Type IV","Glomerular Basement Membrane","Mice"],"keywords":["Perlecan","Basement membrane","Laminin","Knockout mouse","Type IV collagen","Glomerular basement membrane","Immunostaining","Biology","Cell biology","Immunogold labelling","Pathology","Chemistry","Molecular biology","Kidney","Extracellular matrix","Immunohistochemistry","Ultrastructure","Anatomy","Biochemistry","Endocrinology","Immunology","Proteoglycan","Gene","Glomerulonephritis","Medicine"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T12:33:38.996435Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}