{"doi":"10.14336/ad.2021.0621","title":"Identification of Molecules Responsible for Therapeutic Effects of Extracellular Vesicles Produced from iPSC-Derived MSCs on Sjo¨gren’s Syndrome","abstract":"Recent research indicated that extracellular vesicles (EVs) derived from mesenchymal stem/stromal cells (MSCs) are a promising alternative to MSCs for immunomodulatory therapy. However, the contents of MSC-EVs would change as their parent MSCs change, hence the therapeutic efficacy of MSC-derived EVs (MSC-EVs) would largely depend on donors, tissue sources and culture conditions of MSCs. To overcome limitations of tissue-derived MSCs, we previously used MSCs derived from human induced pluripotent stem cells (iMSCs) to produce EVs and demonstrated their therapeutic potential in a mouse model of secondary Sjo¨gren's Syndrome. Here, we further found that EVs from early-passage iMSCs had better immunomodulatory potency than EVs from late-passage iMSCs in TLR4-stimulated splenocytes and in a mouse model of primary Sjögren's syndrome. Comparative molecular profiling using proteomics and microRNA sequencing revealed distinctive molecular profiles of iMSC-EVs with or without immunomodulation capacity. Amongst them, manipulation of TGF-β1, miR-21 and miR-125b levels in iMSC-EVs significantly affected their immunosuppressive effects. These findings would help improve our understanding of the molecular mechanism underlying iMSC-EV-mediated immunomodulation and further provide strategies to improve regulatory function of EVs for the treatment of immune-mediated diseases.","journal":"Aging and Disease","year":2021,"id":158647,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":45,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9512,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":470249,"name":"Qingguo Zhao","orcid":"0000-0002-4861-0735","position":1,"is_corresponding":false},{"id":261408,"name":"Heather Barreda","orcid":null,"position":2,"is_corresponding":false},{"id":259923,"name":"Gagandeep Kaur","orcid":"0000-0003-4227-2306","position":3,"is_corresponding":false},{"id":470251,"name":"Bo Hai","orcid":"0000-0002-7099-8086","position":4,"is_corresponding":false},{"id":259926,"name":"Jong Min Choi","orcid":"0000-0002-5282-7697","position":5,"is_corresponding":false},{"id":259925,"name":"Sung Yun Jung","orcid":"0000-0003-1521-7977","position":6,"is_corresponding":false},{"id":648391,"name":"Fei Liu","orcid":"0009-0005-0576-440X","position":7,"is_corresponding":false},{"id":259928,"name":"Ryang Hwa Lee","orcid":"0000-0001-7112-3583","position":8,"is_corresponding":false},{"id":259921,"name":"Hyemee Kim","orcid":"0000-0002-0713-3952","position":0,"is_corresponding":true}],"reference_count":55,"raw_metadata":null,"created_at":"2026-07-18T23:44:30.791367Z","pmid":"34527418","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}