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Cross-binding assays indicated minimal cross-epitope recognition by nAbs from other herpesviruses, while several potential vulnerable sites on HHV-6B gB were identified. These insights advance our understanding of HHV-6B infection mechanisms and support future development of antibodies or vaccines targeting gB.</jats:p>","journal":"PLOS Pathogens","year":2025,"id":642135,"datarank":0.20794415416798362,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.0,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1669987,"name":"Xin-Yan Fang","orcid":null,"position":1,"is_corresponding":false},{"id":1669988,"name":"Yuan-Tao Liu","orcid":null,"position":2,"is_corresponding":false},{"id":1669989,"name":"Xian-Shu Tian","orcid":null,"position":3,"is_corresponding":false},{"id":1669990,"name":"Lan-Yi Zhong","orcid":null,"position":4,"is_corresponding":false},{"id":1669991,"name":"Pei-Huang Wu","orcid":null,"position":5,"is_corresponding":false},{"id":475473,"name":"Hang Zhou","orcid":"0000-0001-8662-9376","position":6,"is_corresponding":false},{"id":1669992,"name":"Peng-Lin Li","orcid":null,"position":7,"is_corresponding":false},{"id":1669993,"name":"Yan-Lin Yang","orcid":null,"position":8,"is_corresponding":false},{"id":1669994,"name":"Zi-Ying Jiang","orcid":null,"position":9,"is_corresponding":false},{"id":1669995,"name":"Sen-Fang Sui","orcid":null,"position":10,"is_corresponding":false},{"id":426564,"name":"Zheng Liu","orcid":"0000-0002-0405-2348","position":11,"is_corresponding":false},{"id":1654255,"name":"Mu-Sheng Zeng","orcid":null,"position":12,"is_corresponding":false},{"id":1485799,"name":"Cong Sun","orcid":"0000-0003-1786-7420","position":13,"is_corresponding":false},{"id":1669986,"name":"Chu Xie","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Human herpesvirus 6B glycoprotein B postfusion structure, vulnerability mapping, and receptor recognition","abstract":"<jats:p>Human herpesvirus 6B (HHV-6B), a β-herpesvirus that significantly threatens immunocompromised individuals, currently lacks targeted antiviral therapies or vaccines. Glycoprotein B (gB), the primary mediator of membrane fusion during viral entry, is a key target for neutralizing antibody (nAb) and vaccine development. In this study, we determined a 2.8 Å cryo-EM structure of the HHV-6B gB ectodomain in its postfusion conformation, unveiling unique N-terminal features and resolving the furin site for the first time in herpesviruses. Comparative analyses highlighted similarities between HHV-6B gB and gB from human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV), mapping conserved residues across herpesviruses. Cross-binding assays indicated minimal cross-epitope recognition by nAbs from other herpesviruses, while several potential vulnerable sites on HHV-6B gB were identified. These insights advance our understanding of HHV-6B infection mechanisms and support future development of antibodies or vaccines targeting gB.</jats:p>","is_dataset_classified":null,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"40632757","pmcid":"PMC12240383","openalex_id":"https://openalex.org/W4412124590","authors":[],"funders":[{"funder_name":"National Key Research and Development Program of China","grant_id":"2022YFC3400900","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"82030046","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"82402614","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"82070329","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"32241028","title":null},{"funder_name":"Postdoctoral Fellowship Program of CPSF","grant_id":"GZB20230886","title":null},{"funder_name":"China Postdoctoral Science Foundation","grant_id":"2023M743998","title":null},{"funder_name":"China Postdoctoral Science Foundation","grant_id":"2024T171080","title":null},{"funder_name":"Fundamental Research Funds for the Central Universities","grant_id":"84000-31610027","title":null},{"funder_name":"Fundamental Research Funds for the Central Universities, Sun Yat-sen University","grant_id":"24qnpy278","title":null}],"total_grants":10,"fwci":2.2179,"citation_percentile":0.88273119,"influential_citations":0,"citation_trend":[{"year":2025,"count":1},{"year":2026,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1013300&type=printable","host_type":"journal"},{"url":"https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1013300&type=printable","host_type":"publisher"},{"url":"https://dx.plos.org/10.1371/journal.ppat.1013300","host_type":"publisher"},{"url":"https://doi.org/10.1371/journal.ppat.1013300","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/40632757","host_type":"repository"},{"url":"https://doaj.org/article/7b673fb3eadc42758b70697559eced47","host_type":"repository"},{"url":"https://doaj.org/article/e267dd26bd9049c8824fe6d39edab0cc","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/12240383","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC12240383","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC12240383?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Cytomegalovirus and herpesvirus research","Viral-associated cancers and disorders","Herpesvirus Infections and Treatments","Herpesvirus 6, Human","Humans","Viral Envelope Proteins","Antibodies, Neutralizing","Antibodies, Viral","Cryoelectron Microscopy","Roseolovirus Infections","Virus Internalization","Protein Conformation","Cytomegalovirus"],"mesh_terms":["Antibodies, Viral","Cytomegalovirus","Humans","Protein Conformation","Viral Envelope Proteins","Herpesvirus 6, Human","Roseolovirus Infections","Cryoelectron Microscopy","Virus Internalization","Antibodies, Neutralizing"],"keywords":["Ectodomain","Virology","Biology","Epitope","Furin","Herpesvirus glycoprotein B","Neutralizing antibody","Antibody","Glycoprotein","Viral entry","Virus","Receptor","Viral replication","Immunology","Genetics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"uniprot"},{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-07T21:41:27.870037Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}