{"doi":"10.1371/journal.ppat.1012936","title":"A ribosome-interacting jumbophage protein associates with the phage nucleus to facilitate efficient propagation","abstract":"Bacteriophages must hijack the gene expression machinery of their bacterial host to efficiently replicate. Recently, we have shown that the early-expressed protein gp014 of Pseudomonas nucleus-forming phage phiKZ forms a stable complex with the host ribosomes and modulates the overall protein expression profile during phage infection. Here, we discover a nucleus-forming phage, designated Churi, that is closely related to phiKZ. Churi encodes gp335, a homolog of gp014-phiKZ, which is expressed during the early stages of infection, and its overexpression in bacterial cells interferes with bacterial growth, suggesting its role in phage-host interplay. We predict experimentally that gp335 also interacts with host ribosomal proteins, similar to its homolog gp014-phiKZ, thereby strengthening its involvement in protein translation during phage infection. We further show that GFP-tagged gp335 specifically localizes by clustering around the phage nucleus and remains associated with it throughout the infection cycle. The CRISPR-Cas13-mediated deletion of gp335 reveals that the mutant phage fails to replicate efficiently, resulting in an extended latent period. Altogether, our study demonstrates that gp335 is an early-expressed protein of the Chimallivirus Churi that localizes in proximity to the phage nucleus, likely serving a role in localized translation to ensure efficient phage propagation.","journal":"PLoS Pathogens","year":2025,"id":529758,"datarank":0.31191623125197543,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.0,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9594,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1409099,"name":"Sucheewin Krobthong","orcid":"0000-0003-4428-4140","position":1,"is_corresponding":false},{"id":341569,"name":"Chase J. Morgan","orcid":"0000-0002-7498-8678","position":2,"is_corresponding":false},{"id":809185,"name":"Emily G. Armbruster","orcid":"0000-0003-0377-1209","position":3,"is_corresponding":false},{"id":1409100,"name":"Milan Gerovac","orcid":"0000-0002-6929-7178","position":4,"is_corresponding":false},{"id":1409101,"name":"Yodying Yingchutrakul","orcid":"0000-0003-0701-4205","position":5,"is_corresponding":false},{"id":820453,"name":"Patompon Wongtrakoongate","orcid":"0000-0001-6070-1843","position":6,"is_corresponding":false},{"id":28508,"name":"Jörg Vogel","orcid":"0000-0003-2220-1404","position":7,"is_corresponding":false},{"id":635957,"name":"Chanat Aonbangkhen","orcid":"0000-0002-7378-1341","position":8,"is_corresponding":false},{"id":459026,"name":"Poochit Nonejuie","orcid":"0000-0002-5130-1749","position":9,"is_corresponding":false},{"id":244710,"name":"Joe Pogliano","orcid":"0000-0001-8989-2655","position":10,"is_corresponding":false},{"id":459027,"name":"Vorrapon Chaikeeratisak","orcid":"0000-0003-2793-5394","position":11,"is_corresponding":false},{"id":1232545,"name":"Wichanan Wannasrichan","orcid":"0009-0006-4914-3389","position":0,"is_corresponding":true}],"reference_count":52,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:51:01.235017Z","pmid":"39992933","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}