{"doi":"10.1371/journal.ppat.1008877","title":"Genome-wide transposon mutagenesis of paramyxoviruses reveals constraints on genomic plasticity","abstract":"The antigenic and genomic stability of paramyxoviruses remains a mystery. Here, we evaluate the genetic plasticity of Sendai virus (SeV) and mumps virus (MuV), sialic acid-using paramyxoviruses that infect mammals from two Paramyxoviridae subfamilies (Orthoparamyxovirinae and Rubulavirinae). We performed saturating whole-genome transposon insertional mutagenesis, and identified important commonalities: disordered regions in the N and P genes near the 3' genomic end were more tolerant to insertional disruptions; but the envelope glycoproteins were not, highlighting structural constraints that contribute to the restricted antigenic drift in paramyxoviruses. Nonetheless, when we applied our strategy to a fusion-defective Newcastle disease virus (Avulavirinae subfamily), we could select for F-revertants and other insertants in the 5' end of the genome. Our genome-wide interrogation of representative paramyxovirus genomes from all three Paramyxoviridae subfamilies provides a family-wide context in which to explore specific variations within and among paramyxovirus genera and species.","journal":"PLoS Pathogens","year":2020,"id":108749,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9496,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":520537,"name":"Shannon M. Beaty","orcid":"0000-0002-5006-063X","position":1,"is_corresponding":false},{"id":291520,"name":"Christian S. Stevens","orcid":"0000-0003-2472-6984","position":2,"is_corresponding":false},{"id":520538,"name":"Sohui T. Won","orcid":"0000-0002-4821-0854","position":3,"is_corresponding":false},{"id":520539,"name":"Arnold Park","orcid":"0000-0001-8916-2230","position":4,"is_corresponding":false},{"id":103716,"name":"David Sachs","orcid":"0000-0002-4445-2055","position":5,"is_corresponding":false},{"id":520540,"name":"Patrick Hong","orcid":"0000-0002-3701-1497","position":6,"is_corresponding":false},{"id":110494,"name":"Benhur Lee","orcid":"0000-0003-0760-1709","position":7,"is_corresponding":false},{"id":520541,"name":"Patricia A. Thibault","orcid":"0000-0002-7313-0848","position":8,"is_corresponding":false},{"id":291521,"name":"Satoshi Ikegame","orcid":"0000-0002-8183-3527","position":0,"is_corresponding":true}],"reference_count":36,"raw_metadata":null,"created_at":"2026-07-18T23:12:46.854423Z","pmid":"33035269","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}