{"doi":"10.1371/journal.pone.0301989","title":"Quantifying Y chromosome loss in primary and metastatic prostate cancer by chromosome painting","abstract":"Somatic Y chromosome loss in hematopoietic cells is associated with higher mortality in men. However, the status of the Y chromosome in cancer tissue is not fully known due to technical limitations, such as difficulties in labelling and sequencing DNA from the Y chromosome. We have developed a system to quantify Y chromosome gain or loss in patient-derived prostate cancer organoids. Using our system, we observed Y chromosome loss in 4 of the 13 (31%) patient-derived metastatic castration-resistant prostate cancer (mCRPC) organoids; interestingly, loss of Yq (long arm of the Y chromosome) was seen in 38% of patient-derived organoids. Additionally, potential associations were observed between mCRPC and Y chromosome nullisomy. The prevalence of Y chromosome loss was similar in primary and metastatic tissue, suggesting that Y chromosome loss is an early event in prostate cancer evolution and may not a result of drug resistance or organoid derivation. This study reports quantification of Y chromosome loss and gain in primary and metastatic prostate cancer tissue and lays the groundwork for further studies investigating the clinical relevance of Y chromosome loss or gain in mCRPC.","journal":"PLoS ONE","year":2024,"id":465402,"datarank":0.24141568686511508,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.0,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9558,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1205141,"name":"Romina Ghale","orcid":null,"position":1,"is_corresponding":false},{"id":294444,"name":"Subhiksha Nandakumar","orcid":"0000-0003-0258-3484","position":2,"is_corresponding":false},{"id":305297,"name":"Kalyani Chadalavada","orcid":"0000-0001-9097-3989","position":3,"is_corresponding":false},{"id":368447,"name":"Gwo‐Shu Mary Lee","orcid":"0000-0001-9236-9195","position":4,"is_corresponding":false},{"id":108807,"name":"Konrad H. Stopsack","orcid":"0000-0002-0722-1311","position":5,"is_corresponding":false},{"id":1296468,"name":"Yu Chen","orcid":"0009-0003-4936-5481","position":6,"is_corresponding":false},{"id":302625,"name":"Gouri J. Nanjangud","orcid":"0000-0002-8547-1957","position":7,"is_corresponding":false},{"id":368441,"name":"Goutam Chakraborty","orcid":"0000-0002-4387-2621","position":8,"is_corresponding":false},{"id":63890,"name":"Philip W. Kantoff","orcid":"0000-0001-7275-0597","position":9,"is_corresponding":false},{"id":1296467,"name":"Sai Harisha Rajanala","orcid":"0000-0002-2117-120X","position":0,"is_corresponding":true}],"reference_count":10,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:04:46.201722Z","pmid":"38683764","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}