{"doi":"10.1371/journal.pone.0298080","title":"Generation and characterization of monoclonal antibodies against pathologically phosphorylated TDP-43","abstract":"Inclusions containing TAR DNA binding protein 43 (TDP-43) are a pathological hallmark of frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). One of the disease-specific features of TDP-43 inclusions is the aberrant phosphorylation of TDP-43 at serines 409/410 (pS409/410). Here, we developed rabbit monoclonal antibodies (mAbs) that specifically detect pS409/410-TDP-43 in multiple model systems and FTD/ALS patient samples. Specifically, we identified three mAbs (26H10, 2E9 and 23A1) from spleen B cell clones that exhibit high specificity and sensitivity to pS409/410-TDP-43 peptides in an ELISA assay. Biochemical analyses revealed that pS409/410 of recombinant TDP-43 and of exogenous 25 kDa TDP-43 C-terminal fragments in cultured HEK293T cells are detected by all three mAbs. Moreover, the mAbs detect pS409/410-positive TDP-43 inclusions in the brains of FTD/ALS patients and mouse models of TDP-43 proteinopathy by immunohistochemistry. Our findings indicate that these mAbs are a valuable resource for investigating TDP-43 pathology both in vitro and in vivo.","journal":"PLoS ONE","year":2024,"id":446649,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9647,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":301301,"name":"Tiffany W. Todd","orcid":"0000-0003-4327-6114","position":1,"is_corresponding":false},{"id":642238,"name":"Wei Shao","orcid":"0000-0002-8782-4904","position":2,"is_corresponding":false},{"id":1056893,"name":"Caroline J. Jones","orcid":null,"position":3,"is_corresponding":false},{"id":882105,"name":"Kexin Huang","orcid":"0009-0001-5697-6710","position":4,"is_corresponding":false},{"id":238163,"name":"Lillian M. Daughrity","orcid":"0000-0003-4644-7265","position":5,"is_corresponding":false},{"id":239293,"name":"Mei Yue","orcid":null,"position":6,"is_corresponding":false},{"id":897520,"name":"Udit Sheth","orcid":"0000-0003-4360-7892","position":7,"is_corresponding":false},{"id":238179,"name":"Tania F. Gendron","orcid":"0000-0002-7335-2627","position":8,"is_corresponding":false},{"id":238155,"name":"Mercedes Prudencio","orcid":"0000-0002-4894-4858","position":9,"is_corresponding":false},{"id":91831,"name":"Björn Oskarsson","orcid":"0000-0002-1725-9866","position":10,"is_corresponding":false},{"id":54753,"name":"Dennis W. Dickson","orcid":"0000-0001-7189-7917","position":11,"is_corresponding":false},{"id":228616,"name":"Leonard Petrucelli","orcid":"0000-0003-2959-129X","position":12,"is_corresponding":false},{"id":241295,"name":"Yong‐Jie Zhang","orcid":"0000-0002-9900-4313","position":13,"is_corresponding":false},{"id":1056892,"name":"Paula Castellanos Otero","orcid":null,"position":0,"is_corresponding":true}],"reference_count":28,"raw_metadata":null,"created_at":"2026-07-19T02:01:54.980444Z","pmid":"38635657","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}