{"doi":"10.1371/journal.pone.0289395","title":"Cold temperature induces a TRPM8-independent calcium release from the endoplasmic reticulum in human platelets","abstract":"The detection of temperature by the human sensory system is life-preserving and highly evolutionarily conserved. Platelets are sensitive to temperature changes and are activated by a decrease in temperature, akin to sensory neurons. However, the molecular mechanism of this temperature-sensing ability is unknown. Yet, platelet activation by temperature could contribute to numerous clinical sequelae, most importantly to reduced quality of ex vivo-stored platelets for transfusion. In this multidisciplinary study, we present evidence for the expression of the temperature-sensitive ion channel transient receptor potential cation channel subfamily member 8 (TRPM8) in human platelets and precursor cells. We found the TRPM8 mRNA and protein in MEG-01 cells and platelets. Inhibition of TRPM8 prevented temperature-induced platelet activation and shape change. However, chemical agonists of TRPM8 did not seem to have an acute effect on platelets. When exposing platelets to below-normal body temperature, we detected a cytosolic calcium increase which was independent of TRPM8 but was completely dependent on the calcium release from the endoplasmic reticulum. Because of the high interindividual variability of TRPM8 expression, a population-based approach should be the focus of future studies. Our study suggests that the cold response of platelets is complex and TRPM8 appears to play a role in early temperature-induced activation of platelets, while other mechanisms likely contribute to later stages of temperature-mediated platelet response.","journal":"PLoS ONE","year":2024,"id":448377,"datarank":0.26876392038420827,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.0,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9522,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":478633,"name":"Olga T. Filippova","orcid":"0000-0002-8829-3439","position":1,"is_corresponding":false},{"id":954333,"name":"Tahsin Özpolat","orcid":"0000-0001-7527-3933","position":2,"is_corresponding":false},{"id":685569,"name":"Daire Byrne","orcid":null,"position":3,"is_corresponding":false},{"id":685568,"name":"S. Lawrence Bailey","orcid":null,"position":4,"is_corresponding":false},{"id":234996,"name":"Aastha Chauhan","orcid":"0000-0002-4001-4031","position":5,"is_corresponding":false},{"id":368650,"name":"Molly Y. Mollica","orcid":"0000-0002-5975-3539","position":6,"is_corresponding":false},{"id":543811,"name":"J. Harris","orcid":null,"position":7,"is_corresponding":false},{"id":1172557,"name":"Kali Esancy","orcid":"0000-0001-5791-5189","position":8,"is_corresponding":false},{"id":299569,"name":"Junmei Chen","orcid":"0000-0001-6702-2763","position":9,"is_corresponding":false},{"id":1172558,"name":"Ajay Dhaka","orcid":"0000-0001-5783-8582","position":10,"is_corresponding":false},{"id":366670,"name":"Nathan J. Sniadecki","orcid":"0000-0001-6960-4492","position":11,"is_corresponding":false},{"id":299571,"name":"José A. López","orcid":"0000-0002-2478-1767","position":12,"is_corresponding":false},{"id":354143,"name":"Moritz Stolla","orcid":"0000-0002-2866-1674","position":13,"is_corresponding":false},{"id":995524,"name":"Anastasiia Stratiievska","orcid":"0000-0002-5523-0773","position":0,"is_corresponding":true}],"reference_count":61,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:02:08.018689Z","pmid":"38437228","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}