{"doi":"10.1371/journal.pone.0280674","title":"Pod-based e-liquids impair human vascular endothelial cell function","abstract":"Pod-based electronic (e-) cigarettes more efficiently deliver nicotine using a protonated formulation. The cardiovascular effects associated with these devices are poorly understood. We evaluated whether pod-based e-liquids and their individual components impair endothelial cell function. We isolated endothelial cells from people who are pod users (n = 10), tobacco never users (n = 7), and combustible cigarette users (n = 6). After a structured use, pod users had lower acetylcholine-mediated endothelial nitric oxide synthase (eNOS) activation compared with never users and was similar to levels from combustible cigarette users (overall P = 0.008, P = 0.01 pod vs never; P = 0.96 pod vs combustible cigarette). The effects of pod-based e-cigarettes and their constituents on vascular cell function were further studied in commercially available human aortic endothelial cells (HAECs) incubated with flavored JUUL e-liquids or propylene glycol (PG):vegetable glycerol (VG) at 30:70 ratio with or without 60 mg/mL nicotine salt for 90 min. A progressive increase in cell death with JUUL e-liquid exposure was observed across 0.0001-1% dilutions; PG:VG vehicle with and without nicotine salt induced cell death. A23187-stimulated nitric oxide production was decreased with all JUUL e-liquid flavors, PG:VG and nicotine salt exposures. Aerosols generated by JUUL e-liquid heating similarly decreased stimulated nitric oxide production. Only mint flavored e-liquids increased inflammation and menthol flavored e-liquids enhanced oxidative stress in HAECs. In conclusion, pod e-liquids and their individual components appear to impair endothelial cell function. These findings indicate the potential harm of pod-based devices on endothelial cell function and thus may be relevant to cardiovascular injury in pod type e-cigarette users.","journal":"PLoS ONE","year":2023,"id":335886,"datarank":0.8398528864628689,"base_score":2.995732273553991,"endowment":2.995732273553991,"self_citation_contribution":0.4493598410330987,"citation_network_contribution":0.3904930454297702,"self_endowment_contribution":0.4493598410330987,"citer_contribution":0.3904930454297702,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":15,"citers_with_citation_signal":12,"citers_with_endowment":12,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9633,"is_data_producer":true,"deposit_databanks":{"Dryad":["10.5061/dryad.bzkh189dt"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":711177,"name":"Robert M. Weisbrod","orcid":"0000-0002-8297-2975","position":1,"is_corresponding":false},{"id":500879,"name":"Jessica L. Fetterman","orcid":"0000-0002-9286-7538","position":2,"is_corresponding":false},{"id":520726,"name":"Rachel J. Keith","orcid":"0000-0003-3877-0636","position":3,"is_corresponding":false},{"id":464991,"name":"Syed Husain Mustafa Rizvi","orcid":"0000-0003-0136-9646","position":4,"is_corresponding":false},{"id":776917,"name":"Yuxiang Zhou","orcid":"0000-0002-4744-187X","position":5,"is_corresponding":false},{"id":524129,"name":"Leili Behrooz","orcid":"0000-0003-3971-9226","position":6,"is_corresponding":false},{"id":422572,"name":"Rose Marie Robertson","orcid":"0000-0002-8855-0933","position":7,"is_corresponding":false},{"id":355675,"name":"Aruni Bhatnagar","orcid":"0000-0001-6818-0384","position":8,"is_corresponding":false},{"id":293993,"name":"Daniel J. Conklin","orcid":"0000-0002-8385-9982","position":9,"is_corresponding":false},{"id":455411,"name":"Naomi Hamburg","orcid":"0000-0001-5504-5589","position":10,"is_corresponding":false},{"id":711176,"name":"Sana Majid","orcid":"0000-0002-9968-0178","position":0,"is_corresponding":true}],"reference_count":49,"raw_metadata":null,"created_at":"2026-07-19T01:10:02.777338Z","pmid":"36701344","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}