{"doi":"10.1371/journal.pone.0266566","title":"SIRPα - CD47 axis regulates dendritic cell-T cell interactions and TCR activation during T cell priming in spleen","abstract":"The SIRPα-CD47 axis plays an important role in T cell recruitment to sites of immune reaction and inflammation but its role in T cell antigen priming is incompletely understood. Employing OTII TCR transgenic mice bred to Cd47-/- (Cd47KO) or SKI mice, a knock-in transgenic animal expressing non-signaling cytoplasmic-truncated SIRPα, we investigated how the SIRPα-CD47 axis contributes to antigen priming. Here we show that adoptive transfer of Cd47KO or SKI Ova-specific CD4+ T cells (OTII) into Cd47KO and SKI recipients, followed by Ova immunization, elicited reduced T cell division and proliferation indices, increased apoptosis, and reduced expansion compared to transfer into WT mice. We confirmed prior reports that splenic T cell zone, CD4+ conventional dendritic cells (cDCs) and CD4+ T cell numbers were reduced in Cd47KO and SKI mice. We report that in vitro derived DCs from Cd47KO and SKI mice exhibited impaired migration in vivo and exhibited reduced CD11c+ DC proximity to OTII T cells in T cell zones after Ag immunization, which correlates with reduced TCR activation in transferred OTII T cells. These findings suggest that reduced numbers of CD4+ cDCs and their impaired migration contributes to reduced T cell-DC proximity in splenic T cell zone and reduced T cell TCR activation, cell division and proliferation, and indirectly increased T cell apoptosis.","journal":"PLoS ONE","year":2022,"id":282146,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9531,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":724875,"name":"Huan Wang","orcid":"0000-0001-7987-3039","position":1,"is_corresponding":false},{"id":958222,"name":"Francisco Velázquez","orcid":null,"position":2,"is_corresponding":false},{"id":248128,"name":"Gail Newton","orcid":null,"position":3,"is_corresponding":false},{"id":297250,"name":"Charles A. Parkos","orcid":"0000-0002-2490-7657","position":4,"is_corresponding":false},{"id":439594,"name":"Pablo Engel","orcid":"0000-0001-8410-252X","position":5,"is_corresponding":false},{"id":874985,"name":"Daniel Engelbertsen","orcid":"0000-0002-0289-5210","position":6,"is_corresponding":false},{"id":624311,"name":"Andrew H. Lichtman","orcid":null,"position":7,"is_corresponding":false},{"id":266551,"name":"Francis W. Luscinskas","orcid":"0000-0001-7100-4103","position":8,"is_corresponding":false},{"id":957759,"name":"Anu Autio","orcid":"0000-0001-6794-3810","position":0,"is_corresponding":true}],"reference_count":49,"raw_metadata":null,"created_at":"2026-07-19T00:29:15.734856Z","pmid":"35413056","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}