{"doi":"10.1371/journal.pone.0258263","title":"LuNER: Multiplexed SARS-CoV-2 detection in clinical swab and wastewater samples","abstract":"Clinical and surveillance testing for the SARS-CoV-2 virus relies overwhelmingly on RT-qPCR-based diagnostics, yet several popular assays require 2-3 separate reactions or rely on detection of a single viral target, which adds significant time, cost, and risk of false-negative results. Furthermore, multiplexed RT-qPCR tests that detect at least two SARS-CoV-2 genes in a single reaction are typically not affordable for large scale clinical surveillance or adaptable to multiple PCR machines and plate layouts. We developed a RT-qPCR assay using the Luna Probe Universal One-Step RT-qPCR master mix with publicly available primers and probes to detect SARS-CoV-2 N gene, E gene, and human RNase P (LuNER) to address these shortcomings and meet the testing demands of a university campus and the local community. This cost-effective test is compatible with BioRad or Applied Biosystems qPCR machines, in 96 and 384-well formats, with or without sample pooling, and has a detection sensitivity suitable for both clinical reporting and wastewater surveillance efforts.","journal":"PLoS ONE","year":2021,"id":203008,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9578,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":104238,"name":"Allan Gopez","orcid":null,"position":1,"is_corresponding":false},{"id":225873,"name":"Connor A. Tsuchida","orcid":"0000-0002-9563-8052","position":2,"is_corresponding":false},{"id":553737,"name":"Vinson B. Fan","orcid":"0000-0002-1688-7780","position":3,"is_corresponding":false},{"id":620578,"name":"Erica A. Moehle","orcid":"0000-0003-4927-0805","position":4,"is_corresponding":false},{"id":745938,"name":"Lea B. Witkowsky","orcid":"0000-0001-5690-7267","position":5,"is_corresponding":false},{"id":620576,"name":"Jennifer Hamilton","orcid":"0000-0002-7136-1757","position":6,"is_corresponding":false},{"id":245810,"name":"Enrique Lin-Shiao","orcid":"0000-0001-5396-9614","position":7,"is_corresponding":false},{"id":622222,"name":"Matthew McElroy","orcid":null,"position":8,"is_corresponding":false},{"id":620580,"name":"Shana L. McDevitt","orcid":"0000-0003-4372-073X","position":9,"is_corresponding":false},{"id":2372,"name":"Alison Ciling","orcid":"0000-0002-8393-6829","position":10,"is_corresponding":false},{"id":732271,"name":"C. Kimberly Tsui","orcid":"0000-0002-3807-5329","position":11,"is_corresponding":false},{"id":618846,"name":"Kathleen Pestal","orcid":"0000-0001-6886-804X","position":12,"is_corresponding":false},{"id":258498,"name":"Holly K. Gildea","orcid":"0000-0002-9549-890X","position":13,"is_corresponding":false},{"id":745939,"name":"Amanda Keller","orcid":"0000-0002-9605-5836","position":14,"is_corresponding":false},{"id":620581,"name":"Iman Sylvain","orcid":"0000-0001-8929-2801","position":15,"is_corresponding":false},{"id":620582,"name":"Clara Williams","orcid":"0000-0003-1292-6313","position":16,"is_corresponding":false},{"id":745940,"name":"Ariana Hirsh","orcid":"0000-0002-8257-1506","position":17,"is_corresponding":false},{"id":297172,"name":"Alexander J. Ehrenberg","orcid":"0000-0003-4334-9424","position":18,"is_corresponding":false},{"id":225972,"name":"Rose S. Kantor","orcid":"0000-0002-5402-8979","position":19,"is_corresponding":false},{"id":783667,"name":"Matthew Metzger","orcid":"0000-0002-6429-7689","position":20,"is_corresponding":false},{"id":423500,"name":"Kara L. Nelson","orcid":"0000-0001-8899-2662","position":21,"is_corresponding":false},{"id":3664,"name":"Fyodor D. Urnov","orcid":"0000-0001-7542-4084","position":22,"is_corresponding":false},{"id":745941,"name":"Bradley R. Ringeisen","orcid":"0000-0002-1701-5706","position":23,"is_corresponding":false},{"id":620579,"name":"Petros Giannikopoulos","orcid":"0000-0003-4342-1756","position":24,"is_corresponding":false},{"id":2407,"name":"Jennifer A. Doudna","orcid":"0000-0001-9161-999X","position":25,"is_corresponding":false},{"id":620577,"name":"Elizabeth C. Stahl","orcid":"0000-0003-0111-6315","position":0,"is_corresponding":true}],"reference_count":31,"raw_metadata":null,"created_at":"2026-07-18T23:51:14.206397Z","pmid":"34758033","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}