{"doi":"10.1371/journal.pone.0256423","title":"Point-of-care microvolume cytometer measures platelet counts with high accuracy from capillary blood","abstract":"BACKGROUND: Point-of-care (PoC) testing of platelet count (PLT) provides real-time data for rapid decision making. The goal of this study is to evaluate the accuracy and precision of platelet counting using a new microvolume (8 μL), absolute counting, 1.5 kg cytometry-based blood analyzer, the rHEALTH ONE (rHEALTH) in comparison with the International Society of Laboratory Hematology (ISLH) platelet method, which uses a cytometer and an impedance analyzer. METHODS: Inclusion eligibility were healthy adults (M/F) ages 18-80 for donation of fingerprick and venous blood samples. Samples were from a random N = 31 volunteers from a single U.S. site. Samples were serially diluted to test thrombocytopenic ranges. Interfering substances and conditions were tested, including RBC fragments, platelet fragments, cholesterol, triglycerides, lipids, anti-platelet antibodies, and temperature. RESULTS: The concordance between the rHEALTH and ISLH methods had a slope = 1.030 and R2 = 0.9684. The rHEALTH method showed a correlation between capillary and venous blood samples (slope = 0.9514 and R2 = 0.9684). Certain interferents changed platelet recovery: RBC fragments and anti-platelet antibodies with the ISLH method; platelet fragments and anti-platelet antibodies on the rHEALTH; and RBC fragments, platelets fragments, triglycerides and LDL on the clinical impedance analyzer. The rHEALTH's precision ranged from 3.1-8.0%, and the ISLH from 1.0-10.5%. CONCLUSIONS: The rHEALTH method provides similar results with the reference method and good correlation between adult capillary and venous blood samples. This demonstrates the ability of the rHEALTH to provide point-of-care assessment of normal and thrombocytopenic platelet counts from fingerprick blood with high precision and limited interferences.","journal":"PLoS ONE","year":2021,"id":206282,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9523,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":648357,"name":"Rebecca Yu","orcid":"0000-0003-1148-6977","position":1,"is_corresponding":false},{"id":790631,"name":"Helena U. Westergren","orcid":null,"position":2,"is_corresponding":false},{"id":790632,"name":"Jonathan Paraskos","orcid":null,"position":3,"is_corresponding":false},{"id":790633,"name":"Philipp Schatz","orcid":null,"position":4,"is_corresponding":false},{"id":790634,"name":"Anna Tigerström","orcid":null,"position":5,"is_corresponding":false},{"id":790247,"name":"Anna Ekman","orcid":"0009-0003-7649-9899","position":6,"is_corresponding":false},{"id":790248,"name":"José Sánchez","orcid":"0009-0007-2913-1126","position":7,"is_corresponding":false},{"id":790635,"name":"Jamie Cheng","orcid":null,"position":8,"is_corresponding":false},{"id":790249,"name":"Lillian Li","orcid":"0009-0009-1625-0059","position":9,"is_corresponding":false},{"id":790250,"name":"Eugene Chan","orcid":"0000-0003-1065-0050","position":10,"is_corresponding":false},{"id":790630,"name":"William M. Dickerson","orcid":null,"position":0,"is_corresponding":true}],"reference_count":21,"raw_metadata":null,"created_at":"2026-07-18T23:51:37.630221Z","pmid":"34437590","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}