{"doi":"10.1371/journal.pone.0254697","title":"Cell-cell adhesion regulates Merlin/NF2 interaction with the PAF complex","abstract":"The PAF complex (PAFC) coordinates transcription elongation and mRNA processing and its CDC73/parafibromin subunit functions as a tumour suppressor. The NF2/Merlin tumour suppressor functions both at the cell cortex and nucleus and is a key mediator of contact inhibition but the molecular mechanisms remain unclear. In this study we have used affinity proteomics to identify novel Merlin interacting proteins and show that Merlin forms a complex with multiple proteins involved in RNA processing including the PAFC and the CHD1 chromatin remodeller. Tumour-derived inactivating mutations in both Merlin and the CDC73 PAFC subunit mutually disrupt their interaction and growth suppression by Merlin requires CDC73. Merlin interacts with the PAFC in a cell density-dependent manner and we identify a role for FAT cadherins in regulating the Merlin-PAFC interaction. Our results suggest that in addition to its function within the Hippo pathway, Merlin is part of a tumour suppressor network regulated by cell-cell adhesion which coordinates post-initiation steps of the transcription cycle of genes mediating contact inhibition.","journal":"PLoS ONE","year":2021,"id":205046,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9513,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":788423,"name":"Kristina Klupsch","orcid":null,"position":1,"is_corresponding":false},{"id":275199,"name":"Juan A. Osés-Prieto","orcid":"0000-0003-4759-2341","position":2,"is_corresponding":false},{"id":787940,"name":"Selim Chaib","orcid":"0000-0003-0497-2002","position":3,"is_corresponding":false},{"id":787941,"name":"Stephen Henderson","orcid":"0000-0002-9032-3828","position":4,"is_corresponding":false},{"id":787942,"name":"Warren Emmett","orcid":"0009-0002-4739-1515","position":5,"is_corresponding":false},{"id":620968,"name":"Lucy C. Young","orcid":"0000-0003-2703-2347","position":6,"is_corresponding":false},{"id":787943,"name":"Silvia Šurinová","orcid":"0000-0003-0442-9595","position":7,"is_corresponding":false},{"id":788424,"name":"Andreas Blees","orcid":null,"position":8,"is_corresponding":false},{"id":788425,"name":"Anett Pfeiffer","orcid":null,"position":9,"is_corresponding":false},{"id":787944,"name":"Maha Tijani","orcid":"0000-0003-2695-3099","position":10,"is_corresponding":false},{"id":787945,"name":"Fabian Brunk","orcid":"0000-0001-9667-7435","position":11,"is_corresponding":false},{"id":788426,"name":"Nicole Hartig","orcid":null,"position":12,"is_corresponding":false},{"id":787946,"name":"Marta Muñoz-Alegre","orcid":"0000-0003-4775-286X","position":13,"is_corresponding":false},{"id":787947,"name":"Alexander Hergovich","orcid":"0000-0001-7018-5635","position":14,"is_corresponding":false},{"id":787948,"name":"Barbara H. Jennings","orcid":"0000-0003-1696-5256","position":15,"is_corresponding":false},{"id":256139,"name":"Alma L. Burlingame","orcid":"0000-0002-8403-7307","position":16,"is_corresponding":false},{"id":787949,"name":"Pablo Rodriguez‐Viciana","orcid":"0000-0002-8846-543X","position":17,"is_corresponding":false},{"id":788422,"name":"Anne E. Roehrig","orcid":null,"position":0,"is_corresponding":true}],"reference_count":79,"raw_metadata":null,"created_at":"2026-07-18T23:51:30.197308Z","pmid":"34424918","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}