{"doi":"10.1371/journal.pone.0252558","title":"A hybridoma-derived monoclonal antibody with high homology to the aberrant myeloma light chain","abstract":"The identification of antibody variable regions in the heavy (VH) and light (VL) chains from hybridomas is necessary for the production of recombinant, sequence-defined monoclonal antibodies (mAbs) and antibody derivatives. This process has received renewed attention in light of recent reports of hybridomas having unintended specificities due to the production of non-antigen specific heavy and/or light chains for the intended antigen. Here we report a surprising finding and potential pitfall in variable domain sequencing of an anti-human CD63 hybridoma. We amplified multiple VL genes from the hybridoma cDNA, including the well-known aberrant Sp2/0 myeloma VK and a unique, full-length VL. After finding that the unique VL failed to yield a functional antibody, we discovered an additional full-length sequence with surprising similarity (~95% sequence identify) to the non-translated myeloma kappa chain but with a correction of its key frameshift mutation. Expression of the recombinant mAb confirmed that this highly homologous sequence is the antigen-specific light chain. Our results highlight the complexity of PCR-based cloning of antibody genes and strategies useful for identification of correct sequences.","journal":"PLoS ONE","year":2021,"id":202900,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9578,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":783532,"name":"Boya Zhang","orcid":"0000-0003-3458-8220","position":1,"is_corresponding":false},{"id":657370,"name":"Alec A. Desai","orcid":"0000-0002-8322-7096","position":2,"is_corresponding":false},{"id":783533,"name":"Anthony Berardi","orcid":"0000-0002-0442-6274","position":3,"is_corresponding":false},{"id":749334,"name":"Henriette A. Remmer","orcid":"0000-0002-7837-7110","position":4,"is_corresponding":false},{"id":657375,"name":"Peter M. Tessier","orcid":"0000-0002-3220-007X","position":5,"is_corresponding":false},{"id":430006,"name":"Colin F. Greineder","orcid":"0000-0001-9740-7672","position":6,"is_corresponding":false},{"id":657372,"name":"Ghasidit Pornnoppadol","orcid":"0000-0002-0474-2031","position":0,"is_corresponding":true}],"reference_count":33,"raw_metadata":null,"created_at":"2026-07-18T23:51:14.206397Z","pmid":"34634047","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}