{"doi":"10.1371/journal.pone.0246138","title":"Comparing lifeact and phalloidin for super-resolution imaging of actin in fixed cells","abstract":"Visualizing actin filaments in fixed cells is of great interest for a variety of topics in cell biology such as cell division, cell movement, and cell signaling. We investigated the possibility of replacing phalloidin, the standard reagent for super-resolution imaging of F-actin in fixed cells, with the actin binding peptide 'lifeact'. We compared the labels for use in single molecule based super-resolution microscopy, where AlexaFluor 647 labeled phalloidin was used in a dSTORM modality and Atto 655 labeled lifeact was used in a single molecule imaging, reversible binding modality. We found that imaging with lifeact had a comparable resolution in reconstructed images and provided several advantages over phalloidin including lower costs, the ability to image multiple regions of interest on a coverslip without degradation, simplified sequential super-resolution imaging, and more continuous labeling of thin filaments.","journal":"PLoS ONE","year":2021,"id":162659,"datarank":1.241554847969853,"base_score":3.828641396489095,"endowment":3.828641396489095,"self_citation_contribution":0.5742962094733643,"citation_network_contribution":0.6672586384964887,"self_endowment_contribution":0.5742962094733643,"citer_contribution":0.6672586384964887,"corpus_percentile":null,"corpus_rank":null,"citation_count":45,"citer_count":36,"citers_with_citation_signal":28,"citers_with_endowment":28,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9483,"is_data_producer":true,"deposit_databanks":{"Dryad":["10.5061/dryad.xsj3tx9cn"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":571145,"name":"Farzin Farzam","orcid":"0000-0002-9939-1923","position":1,"is_corresponding":false},{"id":571146,"name":"Mohamadreza Fazel","orcid":"0000-0002-6215-1336","position":2,"is_corresponding":false},{"id":571147,"name":"Michael J. Wester","orcid":"0000-0002-3520-7605","position":3,"is_corresponding":false},{"id":571148,"name":"Marjolein B.M. Meddens","orcid":"0000-0002-9965-1342","position":4,"is_corresponding":false},{"id":469105,"name":"Keith A. Lidke","orcid":"0000-0002-9328-4318","position":5,"is_corresponding":false},{"id":569987,"name":"Hanieh Mazloom-Farsibaf","orcid":"0000-0002-2571-0418","position":0,"is_corresponding":true}],"reference_count":41,"raw_metadata":null,"created_at":"2026-07-18T23:45:05.641178Z","pmid":"33508018","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}