{"doi":"10.1371/journal.pone.0236148","title":"Plasma proteomic profiling suggests an association between antigen driven clonal B cell expansion and ME/CFS","abstract":"Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is an unexplained chronic, debilitating illness characterized by fatigue, sleep disturbances, cognitive dysfunction, orthostatic intolerance and gastrointestinal problems. Using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), we analyzed the plasma proteomes of 39 ME/CFS patients and 41 healthy controls. Logistic regression models, with both linear and quadratic terms of the protein levels as independent variables, revealed a significant association between ME/CFS and the immunoglobulin heavy variable (IGHV) region 3-23/30. Stratifying the ME/CFS group based on self-reported irritable bowel syndrome (sr-IBS) status revealed a significant quadratic effect of immunoglobulin lambda constant region 7 on its association with ME/CFS with sr-IBS whilst IGHV3-23/30 and immunoglobulin kappa variable region 3-11 were significantly associated with ME/CFS without sr-IBS. In addition, we were able to predict ME/CFS status with a high degree of accuracy (AUC = 0.774-0.838) using a panel of proteins selected by 3 different machine learning algorithms: Lasso, Random Forests, and XGBoost. These algorithms also identified proteomic profiles that predicted the status of ME/CFS patients with sr-IBS (AUC = 0.806-0.846) and ME/CFS without sr-IBS (AUC = 0.754-0.780). Our findings are consistent with a significant association of ME/CFS with immune dysregulation and highlight the potential use of the plasma proteome as a source of biomarkers for disease.","journal":"PLoS ONE","year":2020,"id":62457,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":51,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9596,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":329990,"name":"Xiaoyu Che","orcid":"0000-0003-0001-4122","position":1,"is_corresponding":false},{"id":329991,"name":"Lucinda Bateman","orcid":"0000-0002-9546-9922","position":2,"is_corresponding":false},{"id":329992,"name":"Aaron M. Cheng","orcid":"0000-0001-7924-075X","position":3,"is_corresponding":false},{"id":227837,"name":"Benjamin A. García","orcid":"0000-0002-2306-1207","position":4,"is_corresponding":false},{"id":310254,"name":"Mady Hornig","orcid":"0000-0001-7572-3092","position":5,"is_corresponding":false},{"id":329993,"name":"Manuel Huber","orcid":"0000-0003-0495-922X","position":6,"is_corresponding":false},{"id":329994,"name":"Nancy G. Klimas","orcid":"0000-0003-1459-3268","position":7,"is_corresponding":false},{"id":330578,"name":"Bohyun Lee","orcid":null,"position":8,"is_corresponding":false},{"id":329995,"name":"Hyoungjoo Lee","orcid":"0000-0003-3348-5270","position":9,"is_corresponding":false},{"id":287391,"name":"Susan Levine","orcid":null,"position":10,"is_corresponding":false},{"id":329996,"name":"José G. Montoya","orcid":"0000-0002-3378-8071","position":11,"is_corresponding":false},{"id":330579,"name":"Daniel L. Peterson","orcid":null,"position":12,"is_corresponding":false},{"id":329997,"name":"Anthony L. Komaroff","orcid":"0000-0001-7672-2518","position":13,"is_corresponding":false},{"id":104439,"name":"W. Ian Lipkin","orcid":"0000-0002-8768-9386","position":14,"is_corresponding":false},{"id":330577,"name":"Milica Milivojevic","orcid":null,"position":0,"is_corresponding":true}],"reference_count":97,"raw_metadata":null,"created_at":"2026-07-18T21:10:11.612909Z","pmid":"32692761","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}