{"doi":"10.1371/journal.pone.0233563","title":"Expression and activation of the steroidogenic enzyme CYP11A1 is associated with IL-13 production in T cells from peanut allergic children","abstract":"Activation of the steroidogenic enzyme CYP11A1 was shown to be necessary for the development of peanut-induced intestinal anaphylaxis and IL-13 production in allergic mice. We determined if levels of CYP11A1 in peripheral blood T cells from peanut-allergic (PA) children compared to non-allergic controls were increased and if levels correlated to IL-13 production and oral challenge outcomes to peanut. CYP11A1 mRNA and protein levels were significantly increased in activated CD4+ T cells from PA patients. In parallel, IL-13 production was significantly increased; IFNγ levels were not different between groups. There were significant correlations between expression levels of CYP11A1 mRNA and levels of IL13 mRNA and protein, levels of serum IgE anti-Ara h 2 and to outcomes of peanut challenge. The importance of CYP11A1 on cytokine production was tested using a CYP11A1 CRISPR/Cas9 KO plasmid or an inhibitor of enzymatic CYP11A1 activity. Inhibition of CYP11A1 activation in patient cells treated with the inhibitor, aminoglutethimide, or CD4+ T cell line transfected with the CYP11A1 KO plasmid resulted in reduced IL-13 production. These data suggest that the CYP11A1-CD4+Tcell-IL-13 axis in activated CD4+ T cells from PA children is associated with development of PA reactions. CYP11A1 may represent a novel target for therapeutic intervention in PA children.","journal":"PLoS ONE","year":2020,"id":80310,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9612,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":267140,"name":"Matthew Strand","orcid":"0000-0002-9083-5989","position":1,"is_corresponding":false},{"id":241615,"name":"Bruce J. Lanser","orcid":"0000-0002-3422-8658","position":2,"is_corresponding":false},{"id":417138,"name":"Carah B. Santos","orcid":null,"position":3,"is_corresponding":false},{"id":416314,"name":"Krešo Bendelja","orcid":"0000-0002-6017-3355","position":4,"is_corresponding":false},{"id":417139,"name":"Jennifer Fish","orcid":null,"position":5,"is_corresponding":false},{"id":417140,"name":"Elizabeth A. Esterl","orcid":null,"position":6,"is_corresponding":false},{"id":417141,"name":"Shigeru Ashino","orcid":null,"position":7,"is_corresponding":false},{"id":416315,"name":"Jordan K. Abbott","orcid":"0000-0001-6334-5266","position":8,"is_corresponding":false},{"id":382543,"name":"Vijaya Knight","orcid":"0000-0002-7514-0097","position":9,"is_corresponding":false},{"id":416316,"name":"Erwin W. Gelfand","orcid":"0000-0002-1256-8918","position":10,"is_corresponding":false},{"id":416313,"name":"Meiqin Wang","orcid":"0009-0004-2102-2206","position":0,"is_corresponding":true}],"reference_count":34,"raw_metadata":null,"created_at":"2026-07-18T21:51:24.704381Z","pmid":"32497050","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}